摘要
探索脑损伤后存活一段时间死亡者脑损伤诊断指标。利用液压冲击装置 ,造成大鼠实验性闭合性脑损伤 ;并用抗 Ca M与 n NOS抗体 ,对鼠脑伤后 3d、1、3、4、8周脑内 Ca M与 n NOS的变化规律进行研究。Ca M与 n NOS染色结果显示 :伤侧鼠脑皮质阳性细胞数、阳性细胞平均灰度、海马平均灰度均较对照组有显著下降 ,在 1周时有回升 ,3、4、8周时有不同的反应。说明通过检测 Ca M与 n
For the purpose of diagnosis of brain injury at the later stage,a closed brain injury rat ,s model has been established by the fluid-percussion.Brain samples were taken 3d,1,3,4,8 weeks after brain injury and were stained by immunohistochemical method for demonstrating CaM and nNOS.The results showed that the positive staining cells in the injured cerebral hemisphere of 3-day group decreased abruptly as compared with the control group,and then increased in the 1-week group.It is suggested that changes of number and of the gray degree of CaM and nNOS positive staining cells can be used to determine the age of the brain injury.
出处
《中国法医学杂志》
CSCD
2000年第2期82-85,共4页
Chinese Journal of Forensic Medicine
