摘要
目的 :探讨甲钴胺是否具有防治氨基甙类抗生素耳毒作用。方法 :用单次肌注大剂量庆大霉素 ( 12 5m g/ kg)造成感音障碍的动物模型。通过系列观察耳蜗电图的变化 ,观察在肌注庆大霉素的同时应用甲钴胺 ( 1m g/ kg)是否具有拮抗庆大霉素的瞬时耳毒作用。结果 :1单次大剂量庆大霉素注射 ,可引起动物复合动作电位CAP N1 ,N2 潜伏期延长与振幅减弱 ;N1 - N2 间期虽有延长 ,变化仍在正常范围内 ;2单次大剂量庆大霉素注射并与甲钴胺同时用药 ,N1 及 N2 的上述变化明显改善。结论 :甲钴胺在增强神经纤维生理功能的同时 ,尚有拮抗庆大霉素的瞬时耳毒作用 ,这可能与甲钴胺在 S-腺苷甲硫胺酸甲基化过程中产生谷胱苷肽 -
Objective:To explore whether is methylcobalamin effective in prophylaxis of AmAn ototoxic action.Method:We attempted to use a single injection of gentamicin(125mg/Kg b.i.w.) to make an animal (guinea pig)model of transient sensory disturbance of hearing, and by a series observation of the changes in ECochG to find out the antagonistic action of methylcobalamin which was simultaneously given to animals(1mg/Kg,i.m.)followed injection of gentamicin.Result:①The transient ototoxic action of gentamicin may cause delay of latency and diminution of amplitude of CAP N 1 and N 2 in animals with a single injection of gentamicin.Though the interval between N 1 and N 2 prolonged,the changes were still within normal ranges.②By contrast,the changes of delay at N 1 and N 2 as mentioned above were significantly improved in animals administered simultaneously with methylcobalamin.Conclusion:It indicated that in addition to the improvement of physiologic function of nerve fibres,methylcobalamin may be also an agent to prevent the transient ototoxic action of gentamicin .Due to methylcobalamin acts as a methyl donor ,it participates the synthesis of SAM (Sadenosylmethionine) and thus products GSH(Glutathion SH),the latter may inhibit ototoxic sideeffects of gentamicin.
出处
《临床耳鼻咽喉科杂志》
CSCD
2000年第7期324-326,共3页
Journal of Clinical Otorhinolaryngology
