摘要
目的研究4.1蛋白家族成员merlin和ezrin在青海藏族胃癌组织中突变与表达特点,同时研究二者对胃癌细胞增殖、黏附侵移能力的影响。方法用PCR及DNA测序检测merlin突变,免疫组化检测ezrin蛋白表达,用MTT方法检测胃癌细胞增殖与黏附侵移。结果青海藏族胃癌组织中检测到merlin蛋白基因NF2外显子17两个位点突变率较高,超过50%。而ezrin蛋白表达在青海藏族胃癌组织中比正常胃黏膜组织中为高(P<0.05),其表达水平与胃癌分化程度、淋巴结转移及组织学分型有关(P<0.05)。此外,MTT检测结果显示,FERM蛋白结构域、merlin-2对胃癌细胞增殖与细胞黏附侵染无明显影响,并且ezrin对胃癌细胞黏附侵移能力亦无明显影响,但对细胞增殖具有一定抑制能力。抑癌基因蛋白merlin-1不但抑制细胞增殖,而且也对细胞黏附侵移具有抑制作用,其中抑癌基因蛋白merlin-1对细胞增殖抑制能力比ezrin蛋白更加明显。结论 Merlin和ezrin蛋白表达与青海藏族胃癌易感性具有一定关系,且两种因子参与调节胃癌细胞增殖和细胞黏附能力。
Objective To study the mutation of tumor suppressor protein merlin and the expression of ezrin in gastric cancer of Qinghai Tibetan patients,meanwhile to explore the influences of merlin and ezrin on the proliferation and adhesion ability of gastric cancer cell.Methods PCR and DNA sequencing were used to analyze the mutation,and the ezrin expression was detected by immunochemical staining.The proliferation and adhesion ability of gastric cancer cell were analyzed by MTT assay.Results The mutation had been detected in merlin gene NF2 exons,and two sites in exon 17 showed high mutation rate,it was more than 50%.Ezrin protein expression was higher in gastric cancer than normal gastric mucosa in Qinghai Tibetan patients（P0.05）.The expression level of ezrin protein was correlated with tumor differentiation,lymph node metastasis and histological type of Qinghai Tibetan patients（P0.05）.Furthermore,MTT assay showed that FERM domain and merlin-2 had no significant influences on the proliferation and adhesion ability of gastric cancer cell,and ezrin showed no effect on the adhesion ability of gastric cancer cell,but it showed ezrin influenced the cell proliferation.Tumor suppressor protein merlin-1 showed not only a much stronger inhibition of proliferation,but also decreased the cell adhesion ability of gastric cancer cell,and the inhibition of proliferation by merlin was much stronger than ezrin protein.Conclusions The expression of merlin and ezrin is related with the susceptibility to gastric cancer development and metastasis in Qinghai Tibetan patients.Furthermore,merlin-1 and ezrin are involved in adjustment of proliferation and adhesion ability of gastric cancer cell.
出处
《基础医学与临床》
CSCD
北大核心
2013年第1期70-76,共7页
Basic and Clinical Medicine
基金
国家自然基金项目(81160259)
青海省科技厅项目(2010-Z-721)
