摘要
目的调查类鼻疽伯克霍尔德氏菌在广西地区的详细分布情况。方法于2007年底对广西地区的14个地级市进行了大规模调查,共采集了77个地点的154个土壤样品和130个水样。采样通过细菌分离培养方法、16S rDNA基因测序和鞭毛基因检测的方法进行鉴定分析,对类鼻疽伯克霍尔德氏菌检出情况的地理分布进行绘图记录。结果 154份土壤样品中分离纯化出13株类鼻疽伯克霍尔德氏菌,阳性率为8.4%,没有在水样中发现该菌;分离纯化结果与16S rDNA基因测序和鞭毛基因检测的结果完全一致。分离纯化类鼻疽伯克霍尔德氏菌阳性的采样点分布在中东、南宁、钦州、放城、北海5个地区。结论广西地区的类鼻疽伯克霍尔德氏菌主要分布在北回归线以南,南海附近地区。
Objective To detect the prevalence and distribution of Burkholderia pseudomallei （B. pseudomallei） in Guangxi province, China, Methods Samples were harvested from several locations in Guangxi region in the year of 2007. A total of 154 soil samples and 130 water samples were screened for B. pseudomallei by bacterial culture, and identification by PCR for species-specific 16S rDNA and flagellin genes. Results B. pseudomallei was detected in 8.4% of the soil samples but in none of the water samples. These results were identical with those from species-specific 16S rDNA and flagellin genes. All positive samples were confined to 5 locations including Zhongdong, Nanning, Qinzhou, Fangcheng and Beihai. Conclusion Almost all positive samples are isolated from places in the south of the Tropic of Cancer and nearby the South China Sea.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2013年第2期142-144,共3页
Journal of Third Military Medical University
基金
国家高技术研究发展计划(863计划
2005AA219070)~~
