摘要
基于Sf2 1昆虫细胞在悬浮培养过程中所表现出的生长代谢特征 ,提出以培养液中残糖浓度作为控制参数 ,并利用限制性基质 (葡萄糖和蛋白水解物 )的间歇补加技术调控细胞生长的方案。实际控制表明 :与批培养相比 ,Sf2 1细胞在两种具代表性的昆虫细胞培养基 (IPL 41和TC 10 0 )中的生长期和稳定期都得到了有效的延长。TC 10 0培养液中最高细胞培养密度由 3 0× 10 6cells/mL提高到 6 5× 10 6cells/mL ;IPL 41培养液中最高细胞培养密度则由 7 0 5× 10 6cells/mL提高到 9 0× 10 6cells/mL。由于限制性基质的间歇补加技术是利用较确定的营养成分来代替复杂昂贵的补料培养基 ,因此更适合于昆虫细胞的大规模高密度培养。
On the basis of the growth and metabolism behavior inherent in suspended Sf21 insect cells,the intermittent feeding of growth limiting nutrients(glucose and protein hydrolysates)was employed in the regulation of cellular growth during the cultivation by using the residual glucose concentration as a reference point.It was shown that as compared with the batch cultivation,the intermittent feeding of growth limiting nutrients effectively prolonged the growth and stationary phase for Sf21 insect cells grown in two representative culture medium(TC 100 and IPL 41).The maximum cell density was increased from 3.0×10 6 cells/ml to 6.5×10 6 cells/mL in TC 100 medium,and in IPL 41 medium,the maximum cell density was increased from 7.05×10 6 cells/mL to 9.0×10 6 cells/mL.As the defined nutrient solution was used for feeding in lieu of the complicated and expensive base medium,the technique would find prospects in large scale high density cultivation of insect cells.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第3期357-362,共6页
Chinese Journal of Biotechnology
关键词
昆虫细胞
悬浮培养
限制性基质
间歇补加
生长
Insect cells
suspension culture
growth limiting nutrients
intermittent feeding
growth
