摘要
目的了解日本血吸虫感染不同动物宿主(包括天然宿主和实验动物宿主)后的虫体发育和宿主组织病理变化差异。方法以黄牛、水牛、山羊、BALB/c小鼠、Wistar大鼠和新西兰大白兔为对象,同一时间人工感染相同来源的日本血吸虫尾蚴,收集感染后相同日龄的虫体(49d),对虫体发育进行比较,对宿主的肝脏病变进行比较,并对黄牛、水牛和山羊的肝脏进行了病理切片的观察和比较。结果不同动物宿主来源的虫体的长度、宽度和发育情况存在着差异,总体来说是适宜宿主体内的虫体长度明显长于非适宜宿主体内的虫体长度,雌虫长于雄虫(Wistar大鼠来源的虫体例外)。发育情况除了Wistar大鼠来源的虫体未发育成熟,其余动物来源虫体均发育成成虫。6种动物宿主的肝脏病变存在很大的差异,适宜宿主感染后肝脏布满虫卵结节,病变尤其严重;而非适宜宿主的肝脏只有少量虫卵结节(水牛),或者几乎看不到虫卵结节(Wistar大鼠)。天然宿主肝脏病理观察发现,黄牛组和山羊组肝脏组织炎性细胞明显增多且聚集,虫卵周围有大量嗜酸性细胞浸润,多量炎性淋巴细胞聚集浸润,形成典型条纹状嗜伊红沉淀物;而水牛组的肝细胞无变性、无炎症细胞的聚集浸润,小叶结构完整。结论日本血吸虫对黄牛、山羊、BALB/c小鼠和新西兰大白兔的易感性高于水牛和Wistar大鼠,对前4种动物的致病性也强于后2种,本文为进一步了解日本血吸虫对不同终末宿主的感染差异提供了一些参考数据。
The aim was to understand the difference for worm development,worm morphology and host＇s histopathology in Schistosoma japonicum（S.japonicum） infected animal hosts,including natural hosts and experimental animal hosts.We artificially infected six animal hosts（yellow cattle,water buffalo,goat,New Zealand rabbit,BALB/c mice,and Wistar rat） with the same source of S.japonicum cercariae.The parasites were perfused through the hepatic portal vein on day 49 after infection.The male and female worms were detached manually and counted,and the length and width of the worms were measured.The results showed that the worm recoveries in permissive hosts were higher than that in non-permissive hosts,and the length of the worms from permissive hosts were greater than those from non-permissive hosts.Parasites in all the hosts could develop into maturation and cause liver damage in their hosts except for Wistar rat.The livers from permissive hosts were fulfilled with white egg nodules,and composed of egg-granulomas;while the livers from water buffalo were red but with few egg nodules;the livers from Wistar rat had no damage at all.The histological sections from livers of infected natural hosts showed that in yellow cattle and goat,hepatocytes displayed mild swelling,and a large number of inflammatory cells were seen to be infiltrating and aggregating,including eosinophils and lymphocytes,and typical striped eosinophilic deposits were observed.Compared with yellow cattle and goat,the structural integrity of the hepatic lobules in infected buffalo was intacted in the livers.There was actinomorphous distribution of hepatic cord centered on central veins,polygonal hepatocytes without edema and inflammatory cell infiltration,leaving only scattered neutrophils and monocytes.This study provides reference data for labora tory and field studies in understanding the differences of S.japonicum infection in different animal hosts.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2012年第12期1207-1211,共5页
Chinese Journal of Zoonoses
基金
国家科技重大专项(2008ZX10004-011
2012ZX10004220-008)
公益性行业(农业)科研专项(200903036)~~
关键词
日本血吸虫
天然宿主
实验动物宿主
发育
病理
Schistosoma japonicum
natural host
experimental animal host
development
pathology
