摘要
用N^+离子注入不同含水量的种子,其胚自由基相对含量与水份含量呈抛物线关系,水份含量在12%—14%之间,自由基含量较高,但水份大于14%时,随水份含量增加,自由基产额下降。种子含水量与发芽率、根长、苗高的损伤率关系均可用指数方程Y=ae^(bx)来描述,但水份含量大于15%时,种子对离子束的敏感性明显增强,自由基含量与发芽率、根长和苗高的损伤率呈负相关,而含水量在15%以下时,自由基含量与上述性状的损伤率呈正相关。N^+离子注入棉子较适宜的含水量为12—14%。
By analysing peroxidase isozyme in second true leaf of M_1 plantsafter N^+ ion implantation into dry cotton seed, the relation of variationof peroxidase isozyme with doses of N^+ ion implantation and damages ofagronomic traits was studied. The results showed that there were distinctchanges in the activity and the numbers of peroxidase isozyme in secondtrue leaf of M_1 plants. In the experiment, 12 zymogram patterns wereobserved. A new 3A band was observed in negative pole and frequency of3A band showed in plants induced by 4×10^(16)N^+/cm^2 dose was the highestin second true leaf treated by 4 doses. The frequencies of 1A and 2A bands disappearing were positivelycorrelated with N^+ ion implantation dose. Seedling height during secondtrue leaf, width of first true leaf, leaf area of first true leaf, boll numberper plant and stem thickness of plants with 3A band were higher thanthose of plants without 3A band. The numbers of isozyme bands weresignficiantly positively correlated with bolls per plant, number of boll-bearing branches and stem thickness. Frequency of zymogram change revealedN^+ ion implantation doses and damaging degree of agronomic traits to acertain extent.
基金
国家自然科学基金
关键词
棉花
离子注入
种子
含水量
辐照
Cotton
Ion implantation
Peroxidase isozyme The subject is sponsored by NSFC
