摘要
对一株临床分离的高粘附力幽门螺杆菌 (Helicobacterpylori ,Hp)菌株M 1进行粘附素基因hpaA的序列分析 ,为研究Hp的粘附提供分子基础。 方法 :设计hpaA的特异引物 ,将PCR产物插入pUC19载体构建hpaA的重组克隆 ,DNA自动分析仪进行序列测定 ,ClustalX和Homology软件分析DNA及其推导出的氨基酸序列。 结果 :构建了粘附素HpaA的重组质粒 pUC hpa ,测序显示hpaA结构基因长 783bp ,开放读框完整 ,无中断 ,与文献报道的序列有差异 ;该基因编码 2 61个氨基酸 ,其中一段氨基酸序列KRTIQK与大肠杆菌粘附素K99、SfaS、CFA/Ⅰ中的涎酸结合位点的结构相似。结论 :Hp粘附素基因hpaA在不同菌株存在差异 ,但其粘附作用有相似的分子基础。
Objective: To study the molecular mechanism of the adhesion of Helicobacter pylori. Methods: The special primers of adhesin gene hpaA of H.pylori were designed and a polymerase chain reaction was performed. Then PCR products were inserted into vector pUC19 and sequenced. Results: A recombinant plasmid pUC hpa was constructed. DNA sequencing showed one open reading frame of 783 bp, which encoded polypeptides of 261 amino acids. The HpaA contains a short sequence of amino acids(KRTIQK) which are similar to those which compose the sialic acid binding motif of E.coli adhesins K99, SfaS and CFA/I. Conclusion: The hpaA gene has differences in sequences between H.pylori strains. But they have similar molecular basis for adhesion to host cells. [
出处
《军事医学科学院院刊》
CSCD
北大核心
2000年第2期91-94,共4页
Bulletin of the Academy of Military Medical Sciences
基金
国家自然科学基金资助项目! (3970 0 0 0 7)
