摘要
利用吸入烹调油烟 (浓度为 43± 4mg/m3)持续染毒制备动物模型 ,以B(a)P气管灌注法染毒 ( 12mg/kg/ 5d)的动物为阳性对照 ,吸入加热的空气 ( 2 4～ 2 8℃ )为阴性对照 ,每组15只动物 ,分 3个时相 ( 2 0 ,40 ,6 0d)观察动物肺组织P53 、bcl-2、bax蛋白表达情况。P53、bax、bcl- 2蛋白表达采用免疫组化法测试 ,结果表明 ,阴性对照组P53 蛋白阳性 ,bcl- 2表达低 ,COF与B(a)P组肺组织有P53 蛋白表达 ,且随染毒时间延长而表达增多 ,以细胞增生、异型性增生病变组织中表达阳性为多。bcl- 2表达与P53 有相似规律 ,bax在组织中表达与bcl- 2相反 。
SD rats were exposed to subacute dose cooking oil fumes (COF) in order to observe p 53 bcl-2, bax protein level of Lung tissues. Rats of test group and negative group inhaled COF(43±4mg/m3×30min/d×20, 40, 60d) and hot air (30min/d×20,40, 80d) respectively,while positive control group was droped B (a) P from trachea(12mg/kg/5d ×20,40,60d). An immunohistochemical study was made to investigate the expression level of P 53 gene. In the study ,the level P 53 of COF and B(a)P group are higher than control group. A close timeeffect relationship was existed in COF group bcl-2 protein level was as like as p 53 protein. The level of p53、bcl-2 protein of adenic metaphasin arcad precancerous lesion tissues were higher than other lung tissues,wheras the level of bax protein was lower than control group. This suggested , the expression of apoptosis related to genes may play role in the pathological changes exposed to COF.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第6期489-490,共2页
Chinese Journal of Public Health
基金
湖南省教委资助项目
