摘要
白腐真菌在环境治理方面有很大的应用潜力.以百里酚作为研究对象,利用白腐真菌在DOX培养基中对其进行微生物降解,通过测定降解体系中木质素过氧化物酶(LiP)、锰过氧化物酶(MnP)、漆酶(Lac)和多酚氧化酶(PPO)的活性,并利用红外光谱仪、气相色谱-质谱分析仪分析了降解产物.结果发现,百里酚在Lac和LjP催化下发生了脱酚基、脱甲基、开环、氧化等作用,得到对应降解产物,从而推测出白腐真菌对百里酚的降解历程.
White rot fungi have great potential applications in environment management. Thymol was degraded by white rot fungi in DOX culture. Enzymes containing lignin peroxidase （LiP）, manganese peroxidase （MnP）, laccase （Lac） and polyphenol oxidase （PPO） were detected in degradation systems, and degraded products were detected by infrared spectrometer and GC - MS analyzer. Results show that Lac and LiP catalyze thymol occur phenolization, demethylation, ring opening and oxidation reactions to receive corresponding degraded products. Thus, degradation mechanism of thymol by white rot fungi is speculated.
出处
《湖南科技大学学报（自然科学版）》
CAS
北大核心
2012年第4期94-97,共4页
Journal of Hunan University of Science And Technology：Natural Science Edition
基金
国家自然科学基金资助项目(50374068)
贵州省科学技术基金项目(黔科合J字[2012]2306)
贵州高层次人才科研条件特助经费项目(TZJF-2011-04)
关键词
白腐真菌
百里酚
降解
机理
white rot fungi
thymol
degradation
mechanism
