摘要
目的 :高密度、高表达培养重组大肠杆菌 TG1/p GEX- h CT,生产重组人降钙素 (rh CT)。 方法和结果 :应用 NBSBio Flo30 0 0型 5 L 自控发酵罐 ,采用分批培养和补料分批培养相结合的培养技术 ,控制碳源和氮源的补加 ,控制溶解氧 ,使重组菌 E.coli TG1/p GEX- h CT发酵光密度 [D(6 0 0 ) ]达到 5 8.6 ,人降钙素和 GST融合蛋白 (GST- h CT)的含量达到 3.2 g/L。结论 :本研究为工业化生产重组 h CT奠定了基础。
Objective: High cell density and high expression culture of recombinant E. coli TG1/pGEX hCT to produce recombinant human calcitonin(hCT). Methods and Results: Batch and fed batch culture were used with the carbon nitrogen control, DO stat method in 5 L NBS BioFlo 3000 type autocontrol fermentor was carried out to produce recombinant hCT in E.coli TG1/pGEX hCT. The final cell density and concentration of GST hCT fusion protein were 58.6 D (600) and 3.2 g/L. Conclusion: This study provides a basic work for production recombinant hCT in industrial scale.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第3期213-215,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金!( 3 9870 170 )
