摘要
目的建立重组GST NK4融合基因工程菌的发酵工艺。方法采用摇瓶、发酵罐发酵,对影响工程菌生长和表达的条件如pH值、诱导时间及诱导剂浓度等进行优化。结果根据优化的条件,15L发酵罐发酵11h ,菌体收获量可达到湿重(2 4 .6±0 .98)g/L ,目的蛋白质的表达量占菌体总蛋白质的5 0 %左右。结论确定了周期短。
PurposeTo develop the best fermentation procedure of E.colli expressing GST-NK4.MethodsOptimizing the the range of pH, induction time and concentration on the collection ratio and expression of the recombinant protein were analyzed with 15 liter fermentation tank.ResultsUnder the established conditions, (24.6±0.98)g/L of wet bacteria could be obtained. And the derivative of recombinant GST-NK4 was about 50% of total protein in the host.ConclusionThe established fermentative procedure increases the collection efficiency and expression of recombinant protein.
出处
《中国生化药物杂志》
CAS
CSCD
2005年第2期102-104,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
NK4
大肠杆菌
发酵
recombinant NK4
E.colli
fermentation
