摘要
目的探讨血清中分泌型蛋白Dickkopf 1(DKK1)在食管癌早期诊断中的应用价值,为食管癌的诊治提供理论参考。方法选取2009年10月至2011年4月在江南大学附属医院就诊患者145例,将其分为4组,食管癌组:72例,男54例、女18例,中位年龄60.4岁;癌前病变组:13例,男9例、女4例,中位年龄58.6岁;良性病变组:30例,男23例、女7例,中位年龄56.4岁;健康组:为体检合格的健康者30例,男19例、女11例,中位年龄58.6岁。采用酶联免疫吸附测定(ELISA)法检测血清中DKK1蛋白水平,并绘制受试者工作特征曲线(ROC)确定阈值。结果食管癌组、癌前病变组血清DKK1蛋白浓度明显高于健康组或良性病变组[(37.5±2.8)μg/L、(19.7±2.7)μg/L vs.(5.7±0.8)μg/L、(6.5±0.8)μg/L,t=47.391,P<0.05];良性病变组患者的血清DKK1蛋白表达水平与健康组比较差异无统计学意义[(6.5±0.8)μg/L vs.(5.7±0.8)μg/L,t=4.215,P=0.374);血清中DKK1蛋白浓度取值为13.4μg/L时,食管癌诊断敏感性为74.1%,特异性为96.7%。结论血清中DKK1蛋白水平升高可能与食管癌的发生、发展有关,并有希望成为食管癌早期诊断的血清学标记物。
Objective To explore the value of serum Dickkopf-related protein 1（DKK1）for the early diagnosis of esophageal cancer,and provide theoretical evidence for the diagnosis and treatment of esophageal cancer.Methods A total of 145 patients who were admitted to the Affiliated Hospital of Jiangnan University between October 2009 and April 2011 were included in this study and divided into four groups.There were 72 patients in the esophageal cancer group（54 males and 18 females with their median age of 60.4 years）,13 patients in the precancerosis group（9 males and 4 females with their median age of 58.6 years）,30 patients in the benign esophageal lesion group（23 males and 7 females with their median age of 56.4 years）,and 30 healthy people in the volunteer group（19 males and 11 females with their median age of 58.6 years）.Serum DKK1 protein concentration was measured by enzyme linked immunosorbent assay（ELISA）.Receiver operating characteristics（ROC） curve was drawn to determine the cutoff level with optimal diagnostic accuracy. Results Serum DKK1 protein levels were significantly higher in the esophageal cancer group and precancerosis group than those in the volunteer group and benign esophageal lesion group [（37.5±2.8） μg/L,（19.7±2.7） μg/L vs.（5.7±0.8） μg/L,（6.5±0.8） μg/L,t=47.391,P0.05].There was no statistical difference in serum DKK1 protein level between the volunteer group and benign esophageal lesion group [（6.5±0.8） μg/L vs.（5.7±0.8） μg/L,t=4.215,P=0.374].When the level of serum DKK1 protein was 13.4 μg/L,the diagnostic sensitivity and specificity of esophageal cancer were 74.1% and 96.7%,respectively.Conclusion Overexpression of serum DKK1 protein could be related to the pathogenesis and progression of esophageal cancer.DKK1 protein may be a potential serologic biomarker for the early diagnosis of esophageal cancer.
出处
《中国胸心血管外科临床杂志》
CAS
2012年第6期659-662,共4页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
