摘要
目的建立气相色谱同时测定血清中甲醇、乙醇、正丙醇含量的方法。方法改变气相色谱条件,以异戊醇为内标,采用气相色谱-氢火焰离子化检测器对血清直接进行检测。并通过待测组分与内标物的响应值比进行定量。结果 GC/FID法检测血清中的甲醇、乙醇、正丙醇含量,得到了良好的线性关系。乙醇浓度从1～100mg/100mL的线性关系式为Y=0.4145X+0.0232(R2=0.9974)、浓度从100～1000mg/100mL的线性关系式为Y=0.4511X+0.0746(R2=0.9911),甲醇浓度从1～200mg/100mL的线性关系式为Y=0.2778X+0.0493(R2=0.9983)。结论该方法操作简便快速,重现性好,通过检测正丙醇还可以推断腐败血样自身产生的乙醇量,是一种较为理想的血醇检测方法。
Objective To develop a method for simultaneous determination of methanol, ethanol and propanol in blood by Gas Chromatography. Methods Conditions of gas chromatography were optimized and then blood serum samples were analyzed quantitatively by GC/FID. Results Linear relations of ethanol were Y= 0. 4145X＋ 0. 0232 （R2 = 0. 9974）for the concentration ranged from 1 to 100mg/100mL and Y= 0. 4511X2-0. 0746 （R2 = 0. 9911）for the concentration ranged from 100 to 1000mg/ 100mL. Linear relation of methanol was Y=0. 2778x＋0. 0493（R2 =0. 9983） for the concentration ranged from 1 to 200mg/ 100mL. Conclusion This method can be applied in clinical diagnosis of poisoning and forensic analysis of alcohol in blood.
出处
《刑事技术》
2012年第6期8-12,共5页
Forensic Science and Technology
基金
中国政法大学青年教师学术(创新团队资助项目
中国政法大学省部级合作项目(No.2011-23210044
2011-23211119)
关键词
甲醇
乙醇
正丙醇
血清
测定
Methanol
Ethanol
Propanol
Serum
Detection
