摘要
目的建立固相萃取-气相色谱法检测生物检材中斑蝥素的方法。方法采用HLB固相萃取柱提取,气相色谱-内标法检测。结果斑蝥素在0.1～200mg/L范围内呈良好的线性关系,相关系数为0.999以上;精密度(RSD)小于5%。对于添加血样,方法检出限为0.05μg/mL,加标回收率范围为93.03%～98.29%。结论该方法操作简便、快速、灵敏,提取回收率高,提取物含杂质较少,可用于实际案件中全血和尿样中斑蝥素的检测。
Objective To develop a solid phase extraction-gas chromatography method for the determination of cantharidin in biological samples.Methods Samples were purified by using HLB solid phase extraction column and detected by gas chromatography with internal standard added.Results The recovery rate of cantharidin in biological samples ranges from 93.03% to 98.29%,the limit of detection is 0.05μg/mL.The method showed excellent linearity（the linear correlation coefficient is 0.9990）.Conclusion The method could be applied in forensic toxicological analysis of cantharidin in blood and urine samples with the advantage of simple and quick operation,high recovery rate,good repetition and clean extraction.
出处
《中国司法鉴定》
北大核心
2011年第2期57-59,共3页
Chinese Journal of Forensic Sciences
基金
证据科学教育部重点实验室开放基金资助项目(09KFKT003)
关键词
斑蝥素
固相萃取
气相色谱法
cantharidin
solid phase extraction
gas chromatography
