摘要
目的:建立超高效液相色谱法测定氯霉素滴眼液中氯霉素的含量和有关物质。方法:采用Acquity UPLC BEH C18(2.1mm×50 mm,1.7μm),流动相为0.01 mol.L-1庚烷磺酸钠缓冲溶液(用磷酸调节pH值至1.5)-甲醇,梯度洗脱,流量为0.5mL.min-1,检测波长为277 nm。结果:氯霉素在43.88～382.4 ng的范围内线性关系良好(r=0.999 3,n=6),平均回收率为99.9%,RSD=0.29%(n=9)。结论:本方法快速、简便、准确、专属性强。
Objective： To establish a UPLC method for content determination and related substances of chloramphenicol eye drops. Method： Acquit uplc BEH C18 column was adopted, mobile phase using 0. 01 mol ·L-1 1-heptanesulfonic acid sodium salt（ adjust pH = 1.5 with phosphoric acid） - methanol as mobile phase ,gradient elute at the flow rate of 0. 5 mL · min-1 and the detection wavelength was 277 nm. Results： The linear response ranges of Chloramphenicol was 43.88-382. 4 ng. （r =0. 999 3, n =6）. The average recovery was 99. 9% （ RSD =0. 29% , n =9）. Conclusion： The method is simple,accurate,sensitive and specific.
出处
《中国药品标准》
CAS
2012年第6期433-436,共4页
Drug Standards of China
