摘要
目的建立超高效液相色谱法测定人血清中多索茶碱浓度。方法建立色谱条件,色谱柱为Waters Acquity C_(18)柱(50mm×2.1mm,1.7μm),流动相为乙腈-水(梯度洗脱),流速为O.2mL·mill^(-1),内标为咖啡因,柱温为室温,检测波长为273nm。结果多索茶碱血药浓度在0.18～23.20 mg·L^(-1)内,与A多索茶碱/A咖啡因之间的线性关系良好,回归方程为Υ=O.181 8χ+0.005 1(r=O.999 9,n=10);日内、日问精密度好(RSD均≤15%),方法回收率为94.70%～106.16%。结论该方法快速、灵敏、准确,可用于多索茶碱血药浓度的测定和药动学研究。
AIM To establish a method for determining the concentration of doxofylline in human serum by ultra high performance liquid chromatography （UPLC）. METHODS The column was Waters Aequity C18（50 mm × 2.1 mm, 1.7μm）, the mobile phase was aeetonitrile-water in gradient programs at flow rate of 0.2 mL·min-1, and the caffeine was used as internal standard. The temperature of cohmm was set at room temperature and the detection wavelength was set at 273 nm. RESULTS The calibration curves were linear in the range of 0.18- 23.20mg· L^-1 for doxofylline; the linear regression equation was y = 0.181 8x ＋ 0.005 1 （ r = 0.999 9, n = 10） ; the RSD of intra-day and inter-day was below 15% ;the recovery rates were 94.70% - 106.16%. CONCLUSION The method is sensitive,rapid and accurate, which is usable for monitoring of doxofylline and pharmacokinetic studies.
出处
《中国临床药学杂志》
CAS
2012年第2期109-112,共4页
Chinese Journal of Clinical Pharmacy
关键词
多索茶碱
超高效液相色谱法
血药浓度监测
doxofylline
ultra high performance liquid chromatography
serum concentration monitoring
