摘要
目的对临床发生慢性排斥反应的肾移植患者及移植术前透析患者的血清中的抗内皮细胞抗体（非HLA抗体）的含量进行定量检测，探讨在肾移植慢性排斥中非HLA抗体的作用。方法采用免疫荧光法对252份血清标本进行抗内皮细胞抗体的检测，包括移植前72例，移植后100例，正常对照组80例。结果移植前透析患者抗内皮细胞抗体的阳性率为38．89％（28／72），其中强阳性占13．89％（10／72），弱阳性占25．00％（18／72）；移植后慢性排斥期患者抗内皮细胞抗体的阳性率为70．00％（70／100），其中强阳性占32．00％（32／100），弱阳性占38．00％（38／100）；正常对照组抗内皮细胞抗体的阳性率为30．00％（24／80），其中强阳性为10．00％（8／80），弱阳性为20．00％（16／80）。移植前与正常对照组抗内皮细胞抗体含量无统计学意义（t=1．0105，P=0．1566），移植前后抗内皮细胞抗体含量差异有统计学意义（t=4．3311，P=0．0000），移植后与正常对照组抗内皮细胞抗体含量差异也具有统计学意义（t=5．5572，P=0．0000）。结论抗内皮细胞抗体作为非HLA抗体是肾移植慢性排斥反应的影响因素之一。
Objective To explore the role of non-HLA antibodies in chronic renal allograft rejection. Methods Immunofluorescence assay was performed to detect anti-endothelial cell antibodies in the serum sam- ples of 252 subjects including 72 patients receiving dialysis before renal transplantation, 100 patients after renal transplantation and 80 normal blood donors. Result Anti-endothelial cell antibodies were detected in 38.89% （28/72） patients before renal transplantation. The strong positive rate accounted for 13.89% （10/72）, and the weak positive rate accounted for 25.00% （ 18/72 ）. Anti-endothelial cell antibodies were detected in 70.00% （70/100） patients after transplantation, in which the strong positive rate accounted for 38.00% （ 38/ 100）, while the weak positive rate accounted for 32.00% （32/100）. Anti-endothelial cell antibodies were detected 30.00% （24/80） in normal blood donors, in which the strong positive rate was 10.00% （8/80） and the weak positive rate was 20.00% （16/80）. The levels of anti-endothelial cell antibodies showed no difference between the pre-transplant group and normal control group （ t = 1. 0105,P = 0. 1566）. The levels of anti- endo- thelial cell antibodies were significantly different between pre-transplant group and post-transplant group （t = 4. 3311 ,P = 0. 0000）. The levels of anti-endothelial cell antibodies showed significant difference between post-transplant group and normal control group （ t = 5. 5572, P = 0. 0000 ）. Conclusion Anti-endothelial cell antibodies were one of the important factors in chronic renal allograft rejection.
出处
《国际免疫学杂志》
CAS
2013年第1期54-56,共3页
International Journal of Immunology
基金
黑龙江省教育厅基金资助项目（11541197）
关键词
肾移植
抗内皮细胞抗体
非HLA抗体
慢性排斥反应
Renal transplantation
Anti-endothelial cell antibody
Non-HLA antibodies
Chronic re- jection reaction
