摘要
以癌胚抗原(CEA)抗体(IgG)作为抗癌药物平阳霉素(A_5)的载体,制备CEAIgG-A_5交联通过胎盘兰排斥试验,乳酸脱氢酶(LDH)释放试验及裸小鼠体内接种治疗,比较CEAIgG-A_5,A_5和CEAIgG对人食管癌细胞株Eca109细胞的体外、体内细胞杀伤作用,结果见到ECAIgG-A_5对Eca109培养细胞杀伤效应大于CEAIgG或A_5(P0.05-0.01);与对照组相比,CEAIgGA_5能明显抑制裸鼠体内Eca-109移植瘤生K,浸润程度降低并趋于高分化,其效应大于A_5,并且乳酸脱氢酶活性明显减弱。
Bleomycin A5was conjugated to anti-CEA/IgG by sodium periodate oxidation method. The cytotoxic effect of the conjugate anti-CEA/IgG-A 5 on human esophageal cancer ECA-109 cell line was studied in comparison with free bteomycin A5 and CEA/IgG atone. The results showed that the cytotoxic effect of the conjugate in vitro and the inhibitory effect on the growth of the transplanted ECA-109 carcinoma in nude mice in vivo were higher than that of bteomycin A 5 or CEA/IgG alone. The LDH release assay used as a cytotoxic index in vitro was discussed .
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1991年第2期93-96,共4页
Chinese Journal of Cancer
