摘要
目的:建立茜草及茜草炭中1,3,6-三羟基-2-甲基蒽醌的UPLC测定方法。方法:采用Acquity BEHC18色谱柱(2.1mm×50mm,1.7μm),流动相为甲醇-0.3%甲酸水,梯度洗脱,流速为0.2mL.min-1,进样量为2μL,柱温30℃,检测波长276nm。结果:1,3,6-三羟基2-甲基蒽醌在1.66～82.98μg.mL-1线性关系良好(r=0.9997),平均回收率为101.47%,RSD=1.92%。结论:该方法简便、快速、准确,为优选茜草炭炮制工艺和建立茜草炭质量标准提供了科学依据。
Objective： To establish an UPLC method for 1, 3, 6-trihydroxy-2-methylanthraquinone in Rubiae Radix et Rhizoma before and after prepared. Methods： An Acquity BEHC18 （2. 1 mm × 50 mm, 1.7 μm） column was used with methanol and 0. 3% formic acid solution as the mobile phase. The flow rate was 0. 2mL.min-1 and volume of injection was 2 μL. Column temperature was 30 ℃： and detective wavelength was 276 nm. Results： There was good liner relationshi Pbetween the peak area and concentration at ranges of 1.66 ~ 82. 98 μg· mL^- 1 （ r = 0. 999 7） for 1, 3, 6-trihydroxy-2-methylanthraquinone. The average recovery rate was 101.47% （RSD = 1.92% ） . Conclusion： The method was rapid and simple with good accuracy and reproducibility for determination of 1, 3, 6- trihydroxy-2-methylanthraquinone in Rubiae Radix et Rhizoma before and after prepared.
出处
《中国现代中药》
CAS
2012年第12期9-11,共3页
Modern Chinese Medicine
基金
中医药行业科研专项(20110700708)
<中国药典>2015年版课题--茜草炭的质量标准研究
关键词
茜草
炒炭
1
3
6-三羟基-2
甲基蒽醌
UPLC
Rubiae Radix et Rhizoma
Carbonizing
1, 3, 6-trihydroxy-2-methylanthraquinone
UPLC
