摘要
目的:探讨自体表皮细胞悬液在修复皮肤组织缺损创面的作用。方法:在20只大鼠身上分别取邮票大小自体全厚皮片进行消化,分离成单个表皮细胞的悬液;经传代培养后,使用自体表皮细胞悬液分别对20只大鼠背部表皮皮肤缺损创面进行覆盖移植,术后观察移植物成活率和植皮区收缩率,同时取移植物进行组织学观察;并与对照组20只自体愈合创面进行相互比较。结果:表皮细胞悬液移植后1周渐融合成片,伤口愈合;经6周后,伤口平整,轻度瘢痕愈合。而对照组在6周时伤口不平整,呈瘢痕愈合,色素沉着明显。结论:在表皮缺损创面上应用体外培养的自体表皮细胞悬液移植修复可达到重构皮肤的目的。
Objective To repair the tissue defect by the Suspension of epidermal cell. Methods a stamp-size normal skin was harvested in forty rats and epidermal ceils suspension was made through decomposing,digestin culturing and re-digesting in lab. Then it Was implanted to the skin defects made on the dorsa of rats. Shrinkage of tt graft survival rate was observed after grafting and samples were taken to make routine paraffin sections f microscopically examination, then compared with autogenous healing. Results After Suspension of epidermal cell was cultured for one week, the wounds healed gradually. The surface of healing wounds was smooth with a slight scar thrc to six weaks later. But the surface of healing wounds of control group was uneven,scar healing and pigmentation was obvious. Conculsion In the skin defect on the application of in vitro culture wound autologous skin cell suspensi（ liquid transplantation to repair the purpose of the reconstruction of the skin.
出处
《中国美容医学》
CAS
2012年第12期2201-2203,共3页
Chinese Journal of Aesthetic Medicine
关键词
移植
组织缺损
表皮细胞悬液
细胞培养
皮肤重建
transplantation
tissue defect
suspension of epidermal cell
cellular culture
reconstruction of human skin
