摘要
目的:①通过点阵CO2激光对兔耳增生性瘢痕治疗的实验研究,验证其有效性;②观察点阵CO2激光治疗兔耳增生性瘢痕后治疗组与对照组成纤维细胞凋亡及VEGF表达情况的变化。方法:新西兰大白兔20只双侧耳制作兔耳增生性瘢痕的动物模型并分组,造模后三周治疗组行点阵CO2激光治疗,对照组无干预。两组切取治疗后1h、治疗后3天、7天、14天、28天瘢痕组织,TUNEL法检测成纤维细胞凋亡率变化,免疫组化检测VEGF的表达变化。结果:点阵CO2激光治疗组瘢痕软化、变平所需时间较对照组缩短,治疗后瘢痕组织中成纤维细胞凋亡增多、VEGF表达减少。结论:点阵CO2激光治疗可以促进兔耳增生性瘢痕软化、变平,瘢痕组织中成纤维细胞凋亡增加、VEGF的表达降低,其治疗增生性瘢痕的机理之一可能在于其启动了增生性瘢痕中过度增殖的成纤维细胞的凋亡程序,加速凋亡,进而影响VEGF的表达,促进瘢痕的萎缩。
Objective To prove the efficacy of fractional C02 laser on hypertrophic scars in the rabbit ear model and analyse the changes of the apoptosis rate of fibroblasts and VEGF secretion after the treatment of fractional CO2 laser on hypertrophic scars. Methods Establish the hypertrophic scar models on rabbit ear in 20 rabbits.Set the treatment group and control group randomly.The treatment group were treated with fractional CO2 laser three weeks after the surgery,the control group got no special treatment.Take photos on day 1,3,7,14,28 after the laser treatment group got the laser treatment and cut off the scar specimen. To detect the the changs of the fibroblasts apoptosis rate with TUNEL and the changs of VEGF with ElivisionTM plus immunohistochemical method. Results The time of hyprotrophic scar became soft and smooth in treatment group is shorter than in control group.Fibroblast apoptosis rate increase and VEGF expression decrease after the laser treatment. Conclusion The fractional CO2 laser can promote the hypertrophic scars become soft and smooth and promote fibroblast apoptosis,decreasing the expression of VEGF on rabbit ear .One of the mechanism of fractional CO2 laser on treating hypertrophic scars in the rabbit ear model perhaps is promote fibroblast apoptosis, decrease the expression of VEGF
出处
《中国美容医学》
CAS
2012年第12期2190-2193,共4页
Chinese Journal of Aesthetic Medicine
