摘要
【目的】探讨缺血预处理对大鼠肝缺血再灌注肾损伤的影响及可能机制。【方法】30只SD大鼠,随机分为假手术对照组(Sham组)、肝缺血再灌注组(IR组)和缺血预处理组(IPC组),每组10例。Sham组仅分离肝门区;IR组用无损伤血管夹夹闭门静脉和肝动脉的分支,阻断肝左、中叶血流,缺血40 min,再灌注6 h;IPC组先缺血5 min,再灌注5 min,然后操作同IR组。肝缺血再灌注6 h,断头处死大鼠,于下腔静脉抽取血样并取左肾和左肝。血样离心测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、尿素氮(BUN)和肌酐(Cr)的含量。左肾一半制成匀浆,测定肾组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的含量,另一半和左肝观察组织病理改变。【结果】与Sham组比较,IR组和IPC组ALT、AST、BUN、Cr、MDA、TNF-α和IL-6含量升高,SOD含量降低(P<0.05),肾组织病理损伤明显;与IR组比较,IPC组ALT、AST、BUN、Cr、MDA、TNF-α和IL-6含量降低,SOD含量升高(P<0.05),肾组织病理损伤减轻。【结论】缺血预处理对肝缺血再灌注导致的肾损伤有保护作用,其保护机制可能与减少氧自由基的释放和抑制炎症反应有关。
[ Objective ] To investigate the effects of ischemic preconditioning on the kidney injury following with hepatic ischemia reperfnsion injury in rats and the mechanism involved. [ Methods ] Thirty healthy male SD rats weighing 200-250 g were randomly assigned into three groups （n = l0 each） ： sham operation group （Sham group）, hepatic ischemia-reperfusion group （IR group） and ischemic preconditioning group （IPC group）. Rats in Sham group underwent surgery, only involved in hepatic hilar region isolating. In IR group, ischemia was achieved by clamping of the branches of the portal vein and hepatic artery that supply the median and left lateral hepatic lobes for 40 min with atraumatic mini-clamp, followed by 6 h reperfusion. In IPC group, each rat was subjected to 5 min ischemia followed by 5 min reperfusion prior to hepatic ischemia, and the rest procedures were as the same as IR group. The animals were killed at 6 h of reperfusion. The left kidney and the left lobe of liver were collected and blood sample was taken from the inferior vena cava. Serum was obtained by centrifugation to measure alanine transaminase （ALT）, aspartate aminotransferase （AST）, blood urea nitrogen （BUN） and creatinine （Cr）. Half of the left kidney was made into homogenate to measure superoxide dismutase （SOD）, malondialdehyde （MDA）, tumor necrosis factor-alpha （TNF-a） and interleukin-6 （IL-6）, while the other and the left lobe of liver were used for pathological analysis. [ Results] Compared with Sham group, the content of ALT, AST, BUN, Cr, TNF-α, 1L-6 and MDA significantly increased, SOD content significantly decreased, and pathological injury was severer in IR group and IPC group. Compared with IR group, the content of ALT, AST, BUN, Cr, TNF-α, IL-6 and MDA significantly decreased, SOD content significantly increased, and pathological injury was milder in IPC group. [ Conclusion ] Ischemic preconditioning can attenuate the kidneyinjury following with hepatic ischemia-reperfusion injury by reducing the oxygen radicals and inhibiting the inflammatory reaction.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2012年第6期761-765,共5页
Journal of Sun Yat-Sen University:Medical Sciences
基金
珠海市科技计划项目(PC20081032)
关键词
再灌注损伤
缺血预处理
氧自由基
超氧化物歧化酶
丙二醛
肿瘤坏死因子-α
白细胞介素-6
reperfusion injury
ischemic preconditioning
reactive oxygen species
superoxide dismutase
malondialdehyde
tumor necrosis factor-alpha
interleukin-6
