摘要
目的 应用新近发现的 p2 7癌基因蛋白单克隆抗体探讨其在肝癌组织中表达的意义。方法 用 p2 7蛋白单克隆抗体对 10 0例肝癌和癌旁肝组织标本进行免疫组化 (S- P法 )染色。结果 (1) p2 7在正常肝组织阳性表达率为 83.3% (5 /6 ) ,癌旁肝炎组织为 84.2 % (32 /38) ,癌旁肝硬化为 5 9.7% ;癌旁肝细胞异型增生为 5 5 .9% (19/34) ,肝癌为 38.0 %。 (2 ) p2 7蛋白在小梁型肝癌 ,高分化肝癌和无转移组阳性表达率分别为 6 3.2 % (2 4/38) ;81.8%(18/2 2 )和 6 7.6 % (2 3/34)。显著高于其他各组 (P<0 .0 1) ,而与肿瘤大体类型无相关性。结论 p2 7癌基因蛋白在肝癌和癌旁肝组织的阳性表达对抑制肝癌细胞的增殖、生长和转移起重要作用 ,可作为肝癌患者预测转移和预后的有用指标。
Objective To investigate the significance of p27 protein expressed in hepatocellular carcinoma (HCC) and pericarcinoma liver tissues.Methods Surgical specimens from 100 cases of hepatocellular carcinoma and pericarcinoma liver tissues were stained using monoclonal antibody of p27 by the S P immunohistochemistry method.Results (1)The positive rates of p27 staining were 83.3%(5/6) in normal liver;84.2%(32/38) in pericarcinoma hepatitis;59.7%(37/62) of pericarcinoma cirrhosis;55.9%(19/34) in liver cell dysplasia;38.0%(38/100) in HCC.(2)The positive rates of p27 were significatly higer in the trabecular HCC(63.2%,24/38),well differentiated HCC(81.8%,18/22) and in those without metastasis (67.6%,23/34).It is not related to morphological types of HCC.Conclusions Expression of p27 in HCC and pericarcinoma tissues may play an important role in suppresion of proliferation,growth and metastasis of HCC,may serve as a marker of predicting metastasis and pognosis of HCC.
出处
《实用肿瘤杂志》
CAS
北大核心
2000年第3期155-157,共3页
Journal of Practical Oncology
基金
福建省卫生厅资助!科研项目 (96 0 36 )
关键词
肝肿瘤
P27蛋白
癌旁组织
基因表达
liver neoplasma/pathology
hepatoma
p27 protein
immunohistochemistry
