摘要
目的：探讨Ａｎｎｅｘｉｎ Ⅰ表达与肺癌及细胞转化的关系。方法：Ｗｅｓｔｅｒｎ印迹、Ｎｏｒｔｈｅｒｎ印迹检测永生化 人支气管上皮 ＢＥＰ２Ｄ细胞及α粒子照射转化后 ＢＥＰ２Ｄ细胞中 Ａｎｎｅｘｎｉ Ⅰ在 ｍＲＮＡ和蛋白质水平的表达，细胞免疫 化学检测 Ａｎｎｅｘｉｎ Ⅰ在 ＢＥＰ２Ｄ细胞中的分布。Ｗｅｓｔｅｒｎ印迹检测肺癌病人正常组织与肿瘤组织中 Ａｎｎｅｘｉｎ Ⅰ蛋白质的 表达。结果： Ａｎｎｅｘｉｎ Ⅰ在α粒子照射转化后 ＢＥＰ２Ｄ细胞中 ｍＲＮＡ和蛋白质水平上的表达光密度均为永生化人支气 管上皮 ＢＥＰ２Ｄ细胞的 １.５倍； Ａｎｎｅｘｉｎ Ⅰ在 ＢＥＰ２Ｄ细胞中分布于胞浆及细胞膜。本研究收集了 ８例肺鳞癌、４例肺腺 癌病人的肺肿瘤组织及正常组织，Ａｎｎｅｘｉｎ Ⅰ在肺癌组织中的表达明显高于正常组织（Ｐ＜０.０１），Ａｎｎｅｘｉｎ Ⅰ在肿瘤 组织中表达的光密度与正常肺组织之比为 ２．７。结论： Ａｎｎｅｘｉｎ Ⅰ在肺癌组织过表达，表明 Ａｎｎｅｘｉｎ Ⅰ可能与肺癌及 细胞恶性转化的发生发展有关。永生化及α粒子照射恶转 ＢＥＰ２Ｄ细胞为进一步研究 Ａｎｎｅｘｉｎ Ⅰ在恶性转化中的作 用提供了良好的模型。
Objective: To evaluate the expression of Annexin I, a calcium-dependent phospholipid binding protein, in relation to lung cancer development and progression, and malignant transformed human bronchial epithiliar cells (BEP2D) by α-particle. Methods: In this study the expression level of Annexin I had been determined by Western blot and Northern blot. The distribution of Annexin I in BEP2D cells was studied by cellular immunochemistry. Results: Northern blot and Western blot showed that the expression level of Annexin I was moderate in immortalized BEP2D cells, but was overexpressed in α-particles transformed BEP2D cells. The amount of expression in transformed BEP2D cells is 1. 5 times as high as in the immortalized ones both in mRNA and protein levels. In BEP2D cell Annexin I distributes on membrane and in plasma, but not in nucleus. However, no difference of distribution between immortalized BEP2D cells and α-particle transformed BEP2D cells was observed. Furthermore, the expression of Annexin I in nontumorous and tumorous lung tissues was determined, which were collected from 4 lung adenocarcinoma and & squamous cell carcinoma patients. Western blotting showed that more Annexin I was expressed in the lung tumorous tissues than in the lung nontumorous tissues, which derived from the same case. The optical density analysis showed that the ratio of expression in the tumorous tissues compared with the nontumorous tissues is 2. 7 (P < 0.01). Conclusions: The expression level of Annexin I is upregulated after cell malignant transformation, and the expression of Annexin I is related to lung cancer. These results suggest that Annexin I overexpression could be a factor implicated in lung tumorigenesis. The model of α particles transformed BEP2D cell line and immortalized BEP2D cell line would offer a novel tool with which to study the function of Annexin I in malignant transformation.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第6期507-511,共5页
Chinese Journal of Cancer
基金
中国博士后基金
国家重点基础专项经费资助!(G1998051208)
