摘要
目的:探讨Annexin Ⅰ在子宫平滑肌瘤中的表达及其与子宫平滑肌瘤发生、发展的关系。方法:选取温州医学院附属第二医院2006-2007年经病理证实的子宫平滑肌瘤标本50例,以及距离包膜2cm以外的瘤旁正常子宫肌层标本50例。应用SP免疫组织化学法检测30例子宫平滑肌瘤及其瘤旁正常子宫肌层组织中Annexin Ⅰ的蛋白水平,应用RT-PCR法检测20例子宫平滑肌瘤及其瘤旁正常子宫肌层组织中Annexin Ⅰ的mRNA水平的表达情况。结果:不管是mRNA水平还是在蛋白水平,正常子宫肌层Annexin Ⅰ高表达,子宫平滑肌瘤组织Annexin Ⅰ低表达,两组间表达差异具有显著性(P<0.01)。结论:Annexin Ⅰ表达下调与子宫平滑肌瘤发生发展密切相关,可作为研究子宫平滑肌瘤发病机制的重要指标。
Objective To investigate the relationship between the expression of Annexin I and tumorigensis and progression in uterine leiomyoma. Methods Fifty uterine leiomyoma tissues and matched normal myometrium tissues situated more than 2 cm away from the leiomyoma capsule from the Second Affiliated Hospital of Wenzhou Medical College between 2006 and 2007 were studied. Thirty cases of leiomyoma tissues and matched normal myometrium tissues were examined by SP immunohistochemiscal staining, while the other 20 cases were examined by RT-PCR, Results The expression of Annexin I in normal myometrium tissues was significantly higher than that in leiomyoma （P 〈 0.01 ） at both mRNA and protein levels, Conclusion Down-regulation of Annexin I may play an important role in the development of leiomyoma, and it can be an significant indicator for studying the pathogenesis of uterine leiomyoma.
出处
《实用医学杂志》
CAS
2008年第17期2942-2944,共3页
The Journal of Practical Medicine
基金
温州市科技计划项目(编号:Y2004A044)
关键词
平滑肌瘤
免疫组织化学
逆转录聚合酶链反应
膜联蛋白Ⅰ
Leiomyoma Immunohistochemistry Reverse transcriptase polymerase chain reaction Annexin I
