摘要
通过对1株和由多株白腐真菌复配共同参与下的复合菌产酶量和达到最高酶活所需时间进行比较,筛选出白腐真菌最佳产酶的复配方式.结果表明,青顶拟多孔菌和糙皮侧耳菌两株菌相互发生协同作用,促进漆酶的分泌,产酶量为50.45U·mL-1;青顶拟多孔菌、糙皮侧耳菌和偏肿拟栓菌这3株菌在分泌过程中促进作用大于抑制作用,产酶量最高为75.98U·mL-1;青顶拟多孔菌、糙皮侧耳菌和血红密孔菌三者相互之间出现拮抗作用,产酶量最低为2.91 U·mL-1;实验证明,在白腐真菌之间既存在拮抗作用,也存在协同作用,因此达到最高酶活所用时间也不同.
In this study,the maximum laccase（Lac） activities by single white-rot fungus and combining white-rot fungi were investigated.The optimal combinations of white-rot fungi were obtained by comparing the maximum Lac activities and the time of achieving the maximum Lac activity.The results showed that the synergy of white-rot fungi Polyporellus picipes and Pleurotus ostreatus improved the secretion of the laccase,with the enzyme production of 50.45 U·mL-1,as well as the combination of white-rot fungi Polyporellus picipes,Pleurotus ostreatus and Pseudotrametes gibbosa promoted the laccase production of 75.98 U·mL-1.However,the combination of white-rot fungi Polyporellus picipes,Pleurotus ostreatus and Pycnoporus sanguineus generated antagonistic effect,with the enzyme production of 2.91 U·mL-1.Both synergistic and antagonistic effects among the white-rot fungi results in different time for achieving the maximum Lac activity of white-rot fungi.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2013年第1期271-276,共6页
Environmental Science
基金
国家自然科学基金项目(21177033)
关键词
白腐真菌
复合菌
酶
漆酶
酶活
white-rot fungi
combining white-rot fungi
enzyme
laccase
enzyme activities
