摘要
目的探讨流式细胞术检测技术在多发性骨髓瘤（MM）患者的诊断意义。方法收集山西医科大学第二临床医学院2009年01月至2011年12月门诊及住院的MM患者28例,检测骨髓瘤细胞表面的免疫表型,分析其表达率与骨髓瘤细胞形态特征及预后相关检测指标的关系。流式抗原表达数、骨髓细胞数组间差异采用卡方和秩和检验。结果流式细胞分析应用CD45/SSC配合CD38＋/CD138＋设门证实MM患者可疑瘤细胞比例为4.0﹪~75.4﹪,形态学检查骨髓瘤细胞比例在为8.0﹪~83.5﹪,骨髓瘤细胞CD138、CD38、CD56、CD19抗原阳性表达比例分别为95.5﹪、89.3﹪、53.6﹪、7.1﹪（c2=54.343,P=0.000）。结论 CD45/SSC配合CD38＋/CD138＋设门技术可以较准确地设定需要分析的MM细胞群;幼稚浆细胞型骨髓瘤易见到CD56阳性表达,流式细胞分析技术与形态学相结合,更有助于MM诊断及鉴别诊断。
Objective To compare the efficiency of flow cytometry and morphological classification of bone marrow smears in the diagnosis of multiple myeloma（MM）.Methods Twenty-eight confirmed MM patients were enrolled.MM cells surface antigens were detected using flow cytometry.The relationship between surface antigen expressions and cell morphological characteristics and laboratory prognosis indices were analyzed.Results Using CD45/SSC and CD38＋/CD138＋ as markers for MM cells,4.0﹪-75.4﹪ of bone marrow cells were MM cells.In comparision,8.0﹪-83.5﹪ cells were considered to be MM cells using morphological examination.The percentages of CD138,CD38,CD56,CD19 positive cells were 95.5﹪,89.3﹪,53.6﹪,7.0﹪ respectively（c2 = 54.343,P = 0.000）.Conclusions The use of CD45/SSC with CD38＋/CD138＋ gating technique can identify multiple myeloma cells,and more immature myeloma cells were seen in the CD56 positive cells.Flow cytometry combined with morphology was helpful to the diagnosis and differentiated diagnosis of multiple myeloma.
出处
《中华细胞与干细胞杂志（电子版）》
2012年第2期24-27,共4页
Chinese Journal of Cell and Stem Cell（Electronic Edition）
