摘要
目的观察平阳霉素(PYM)对体外培养的人脐静脉血管内皮细胞株(ECV304)增殖和细胞周期的影响.方法采用MTT法检测不同浓度PYM(10-2～103μg/m1)作用不同时间(24、48、72 h)后对ECV304细胞的生长抑制率.利用流式细胞仪检测一定浓度PYM刺激下细胞周期分布.结果10μg/ml浓度PYM作用24、48、72 h后的细胞生长抑制率分别为44.7%、59.7%、74.4%,药物抑制效应表现为剂量依赖性和时间依赖性.药物作用24、48、72 h的半数抑制浓度(IC50)分别为32.94、2.56、0.75μg/ml.1μg/ml浓度PYM作用ECV304细胞24 h,实验组S期细胞比例较对照组明显下降,G2-M期细胞比例明显增加(P<0.01),细胞被阻滞在G2-M期.结论PYM对ECV304细胞的增殖具有较强抑制作用,其机制与细胞周期阻滞作用有关.
Objective To study the effect of pingyangmycin (PYM, bleomycin A5) on the proliferation and cell cycle of the cultured ECV304 cells, a human umbilical vein endothelial cell (HUVEC) line. Methods The growth inhibition of PYM on ECV304 cells was measured by MTT assay and the changes in the cell cycle by flow cytometry. Results After 10 μg/ml PYM treatment of the cells for 24, 48, and 72 h, the inhibition rates were 44.7%, 59.7%, and 74.4% respectively, showing a dose- and time-dependent inhibitory effect, with the 50% inhibitory concentration (IC 50 ) of PYM corresponding to treatment durations of 24, 48 and 72 h being 32.94, 2.56 and 0.75 μg/ml respectively. Flow cytometry showed that ECV304 cell cycle was arrested at G 2 -M phase after 24-hour treatment with 1 μg/ml PYM, with significant reduction in the cell ratio of S phase and increase of G 2 -M phase (P<0.01) Conclusion PYM can effectively inhibit the proliferation of ECV304 cells, the mechanism of which might involve the blocking of cell cycle.
出处
《第一军医大学学报》
CSCD
北大核心
2003年第8期830-832,836,共4页
Journal of First Military Medical University
