摘要
目的观察牛珀至宝微丸对肺损伤早期纤维化大鼠转化生长因子β1(TGF-β1)的影响。方法 48只SD大鼠随机均分为4组:对照组(C组)、内毒素组(LPS组)、低剂量牛珀至宝微丸预处理组(LD组)、高剂量牛珀至宝微丸预处理组(HD组)。采用气管内联合腹腔注射内毒素法复制肺损伤早期纤维化模型。实验结束前留取标本测定肺血管通透性(EB值)及肺组织湿/干重量比(W/D);HE染色光镜下观察肺损伤程度(DAD评分);用免疫组化法和蛋白质免疫印迹法测定肺组织TGF-β1蛋白表达;Van Gieson染色法检测肺组织胶原纤维表达。结果与LPS组比较,LD组以及HD组,尤其是HD组的EB、W/D值和DAD评分明显降低(P<0.01),光镜下肺肺损伤及纤维化的程度均减轻,肺组织中TGF-β1表达明显减少(P<0.01)。结论牛珀至宝微丸通过抑制肺组织TGF-β1蛋白的激活,有助于减轻内毒素所致肺损伤早期纤维化。
Objective To observe the effect of Niupo zhibao micropill on expression of trans- forming growth factor beta 1 （TGF-β1） in rats with early fibrosis of pulmonary injury. Methods 48 SD rats were randomly divided into the following 4 groups： the control group （group C） , the endotoxin group （LPS group）, pretreatment group with low dosage Niupo zhibao micropill （LD group）and pre- treatment group with high dosage Niupo zhibao micropill （HD group）. Model of early fibrosis of pul- monary injury was set by intratracheal and intraperitoneal injection of endotoxin. Before experiment was ended, the specimens of 4 groups were collected for assay of pulmonary vascular permeability（EB） and pulmonary tissue wet/dry proportion by weight （W/D） ; degree of pulmonary injury was observed un- der HE microscopy （ DAD score） ; Immunohistochemistry and Western blot method were used for detec- tion of TGF-β1 expression;Van Gieson staining method was used for testing of collagen fibrils expres- sion of pulmonary tissue. Results Compared with LPS group, the EB, W/D ratio and DAD score in LD group and liD group decreased significantly （ P 〈 0.01 ）, degree of pulmonary injury and fibrosis relieved under microscopy, and TGF-β1 expression in pulmonary tissue reduced obviously （P 〈 0. 01 ）. Conclusion Niupo zhibao micropill can suppress activation of TGF-β1 in pulmonary tissue and relieve early fibrosis of pulmonary injury induced by endotoxin.
出处
《实用临床医药杂志》
CAS
2012年第23期1-4,共4页
Journal of Clinical Medicine in Practice
基金
广东省中医药管理管局资助项目(2050069)
关键词
牛珀至宝微丸
肺损伤
肺纤维化
转化生子因子β1
Niupo zhibao micropill
pulmonary injury
pulmonary fibrosis
transforming growth factor beta 1
