摘要
采用微生物平板涂布法对2家城市污水处理厂污水处理各阶段的四环素类药物(四环素、金霉素、土霉素)耐药菌分布进行了测定,分析了污水处理的各阶段对四环素类药物耐药菌的去除效果。结果显示,2家污水处理厂污水处理系统中均能检出土霉素、四环素、金霉素耐药菌,数量分布均为土霉素耐药菌>四环素耐药菌>金霉素耐药菌。3种耐药菌的浓度均在加氯消毒出水中为最小(在0～103 cfu/mL),在外排污泥中最大(高于进水浓度,在105～107 cfu/mL)。四环素耐药菌数从进水的1.1×105～1.6×105 cfu/mL降为出水的0～5.5×102 cfu/mL,说明污水处理过程对四环素耐药菌有一定的去除效果,但四环素耐药菌占总菌数的百分比没有明显下降或上升,相比于进水的1.1%～3.4%,出水仍然保持在3.1%～4.3%左右,表明污水处理过程中没有选择性的富集或去除四环素耐药菌。
Spread plate method was used to determinate the distribution of tetracyclines （TCs） resistant bacteria in two municipal wastewater treatment plant. The performance of different treatment process for TCs resistant bacte- ria removal was investigated. Three TCs-resistant bacteria were detected in both municipal wastewater treatment plants; they were oxytetracycline resistant bacteria, tetracycline resistant bacteria and chlortetracycline resistant bac- teria. The conlony density of three TCs-resistant bacteria followed the order of xytetracycline resistant bacteria〉tet-racycline resistant bacteria〉chlortetracycline resistant bacteria. The colony counts of TCs-resistant bacteria were smallest in chlorination effluent （about 0-10^3 cfu/mL） and highest in waste sludge （about 10^5-10^7 cfu/mL）. The wastewater treatment process had perfect efficiency for tetracycline resistant bacteria removal, the colony counts of tetracycline resistant bacteria in iniluent was decreased from 1.1×10^5～1.6×10^5 cfu/mL to final effluent of 0～5.5×10^2 cfu/mL. While, the percentage of tetracycline resistant bacteria in total TCs-resistant bacteria did not present significantly decline, it was 1.1%-3.4 % in influent water and maintained 3.1%-4.3 % in final effluent water. It could be concluded that the wastewater treatment process was not selective for enrichment or removal of tetracycline resist-ant bacteria.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2012年第12期5-8,共4页
Environmental Pollution & Control
基金
国家自然科学基金资助项目(No.21007010)
教育部博士点基金资助项目(No.20090075120007)
中央高校基本科研基金资助项目
交通运输部科技项目(No.2010353343290)
上海市科学技术委员会科研计划项目(No.09230500200)
湖南省交通厅科研项目(No.200908)
关键词
城市污水厂
四环素类耐药菌
分布特性
municipal wastewater treatment plant
tetracyclines resistant bacteria
distribution characteristics
