摘要
以裂殖酵母NAC蛋白β亚基为对象在禾谷镰刀菌(Fusarium graminearum)基因组数据库中进行搜索,发现了同源蛋白基因FGSG_08675并命名为FgβNAC。通过基因敲除技术对禾谷镰刀菌FgβNAC基因进行敲除和回补,分别获得了敲除菌株fgβnac和回补菌株fgβnac::FgβNAC,并分别对野生型菌株、敲除菌株和回补菌株进行了表型和致病性研究。结果表明,与野生型菌株PH-1相比,敲除菌株fgβnac在固体马铃薯培养基(PDA)上生长速率明显减慢,约为野生型菌株的一半,气生菌丝致密短小;同样在液体PD培养液中培养3 d的敲除突变株的菌丝干重仅为野生型的56%。另外,敲除突变株的分生孢子产量相比野生型菌株下降了78.5%。进一步采用麦穗离体接种法对其致病性进行了检测,发现FgβNAC基因的缺失引起致病力显著地下降,仅局限于接种部位及邻近的小颖,而无法侵入维管束组织引起穗枯症状。
In this study,we found a homologous protein of fission yeast β subunits in the Fusarium graminearum genome database,which is FGSG_08675 and named it FgβNAC.Through the gene knockout technology,we obtained the deletion mutants of the FgβNAC gene in F.graminearum.Further,we obtained the complemented strain of Fgβnac.Compared the wild-type strains,the deletion mutants and the complemented strains,we found that the vegetative growth of the deletion mutants reduced in solid medium PDA and aerial mycelium became denser than PH-1.Conidia produced by the deletion mutants decreased by 78.5% compared with the wild-type strain.In infection assays with wheat spikes,thefgβnacmutant was significantly reduced in virulence.They were limited to theinoculation site and could not invade the vascular tissue to cause ear blight symptoms.
出处
《生物技术通报》
CAS
CSCD
北大核心
2012年第11期177-184,共8页
Biotechnology Bulletin
基金
国家"863"项目(2011AA10A205)
