摘要
本文采用细胞化学方法,对健康和禾谷镰刀菌(Fusarium graminearum)侵染的小麦穗组织中细胞壁主要成分进行了比较分析。电镜观察发现,被侵穗部组织细胞壁中的主要成分如纤维素、木聚糖和果胶质的标记密度下降,显著低于未接种的健康对照组织。结果表明病菌侵染和扩展过程中分泌产生了纤维素酶、木聚糖酶和果胶酶等细胞壁降解酶类,造成寄主细胞壁成分的分解及细胞壁松弛,从而有利于病菌在寄主穗部组织中的侵染和扩展。
The cellulose, xylan and pectin components in cell walls of uninoculated and Fusarium graminearum-infected wheat spikes were localized by means of enzyme-gold and immunogold labeling methods. The observation showed that the labeling densities for cellulose, xylan and pectin in the cell walls of infected wheat spikes were significantly reduced as compared with corresponding cell walls of healthy wheat spikes. These results provide evidence that F. graminearum may produce cell-wall degrading enzymes such as cellulase, xylanase and pectinase during its infection and colonization on wheat spikes.
出处
《植物病理学报》
CAS
CSCD
北大核心
2007年第6期623-628,共6页
Acta Phytopathologica Sinica
基金
国家杰出青年基金项目(30125031)
教育部科学技术研究重点项目(03158)
教育部长江学者和创新团队发展计划项目(IRT0558)
高等学校学科创新引智计划资助项目(B07049)
国家支撑计划项目(2006BAD08A05)
关键词
小麦
禾谷镰刀菌
细胞壁降解酶
细胞化学
wheat
Fusarium graminearum
cell wall-degrading enzymes
cytochemistry
