摘要
用RACE法从中华大节竹幼苗中克隆了K+通道蛋白β亚基,命名为KIB1(基因登录号:JX900132)。序列分析表明,该基因全长1 211 bp,开放读码框为987 bp,编码329个氨基酸多肽。经氨基酸同源性和聚类分析证实,KIB1与拟南芥(KAB1)和水稻(KOB1)钾离子通道蛋白的序列同源性较高,其同源性分别为85.06%和82.32%。亚细胞定位结果表明,该蛋白主要存在于线粒体中(86.1%)。
A bamboo full-length cDNA (named as KIBI ) enconding K + channel β subunit was cloned from In- dosasa sinica using RACE method. Sequence analysis indicated that the full-length cDNA of KIB1 was 1211 bp, containing an open reading frame (ORF) of 987 bp, encoding a deduced polypeptide of 329 amino acids. The de- duced protein shared a high degree of homology with KAB1 ( 85.06 % ) and KOBI ( 82.32 % ). In addition, subcellular localization revealed that this protein mainly located in the mitochondrion (86. 1% ).
出处
《西部林业科学》
CAS
北大核心
2012年第6期15-19,共5页
Journal of West China Forestry Science
基金
西南林业大学博士科研启动基金项目
云南省高校风景园林创新团队(23002802)资助