摘要
为了解小麦品种山融3号耐盐性的基因状况,以大麦HvHAK1为探针,通过电子克隆和PCR方法,从该品种根的cDNA文库中克隆到一个小麦的耐盐相关基因TaHAK1(High Affinity K+transporter1)。该基因ORF全长2 405bp,编码776个氨基酸,含有8个内含子。与已报道的大麦、水稻、玉米等单子叶植物的HAK基因具有较高的相似性。RT-PCR分析表明,TaHAK1在小麦根中表达量较高,且受钾饥饿和盐胁迫诱导表达。在200mmol.L-1 NaCl胁迫条件下,TaHAK1在耐盐小麦品种山融3号中的最高表达量高于在盐敏小麦品种济南177中的最高表达量。这些结果说明,在小麦中克隆到了大麦HvHAK1的同源基因,该基因参与了小麦对高盐、低钾等非生物胁迫的响应,可能对山融3号的耐盐性具有一定贡献。
Using the barley TvHAK1 gene as a probe,we isolated a salinity tolerance related gene from wheat using in silicon cloning and PCR analysis.The full length cDNA of TaHAK1 was obtained from a cDNA library constructed from salt treated wheat roots and was designated as TaHAK1.The open reading frame（ORF） of TaHAK1 was 2 405 bp in length encoding 776 amino acids.There were eight introns in the genomic DNA sequence of TaHAK1 and twelve trans-membrane segments（TMS） in the peptide encoded.Genbank blastp search showed that TaHAK1 had high similarities with other plant HAKs.Semi-quantitative RT-PCR analysis demonstrated that TaHAK1 was mainly expressed in roots and was induced by K＋ starvation and salt stress.Under salt stress,the maximum accumulation of TaHAK1 was higher in Shanrong3 than that in Jinan 177.These results suggested that TaHAK1 may take part in wheat response to salt stress and K＋ starvation and it may play a role in Shanrong3 adaptation to salinity.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2011年第6期1014-1019,共6页
Journal of Triticeae Crops
基金
国家自然基金资助项目(31000710)
农业部转基因生物新品种培育重大专项(2009ZX08009-082B)
济南大学博士基金项目(XBS0851)
