摘要
目的 研究免疫球蛋白对惊厥大鼠的临床作用及其对大鼠脑神经细胞c fos表达的影响。方法 在Wistar大鼠腹腔内注射戊四氮 (pentylenetetrazol,PTZ)诱发大鼠惊厥发作 ,制作癫模型。将 15只Wistar大鼠随机分为 3组 ,A组 :正常对照组 ;B组 :PTZ +静脉注射免疫球蛋白组 ;C组 :PTZ +生理盐水组。观察比较B、C两组每天惊厥情况、采用ABC法观察两组脑神经细胞c fos表达。结果 (1)A组脑神经细胞偶见c fos表达。 (2 )B组大鼠无死亡 ,惊厥潜伏期延长 ,评分降低。而C组大鼠死亡 3只 ,惊厥潜伏期有缩短的趋势 ,评分未降低。两组惊厥评分与潜伏期的的变化趋势不同。 (3)B组比C组脑神经细胞c fos表达低、数量减少 ,经统计学处理 ,差异有显著意义 (P <0 .0 1)。结论静脉注射免疫球蛋白具有一定的抗惊厥作用 ,并使c fos表达降低。
Objective To explore clinical effects of immunoglobulin on convulsion rats and influences of immunoglobulin on the c-fos expression of neurocytes. Methods The epilepsy model was established by injecting pentylenetetrazol (PTZ) into Wistar rats to induce the generalized seizures. Fifteen rats were randomly divided into three groups, that is, group A: normal control; group B: PTZ plus intravenous immunoglobulin (IVIG); group C: PTZ plus normal saline (NS). Seizure conditions of rats were observed and compared between group B and C. All perfused brain tissues of rats were sectioned and detected the c-fos expressions by immunohistochemistry staining (ABC method) at the 14th day of the epilepsy model. Results (1) In group A, weak c-fos expressions in brain tissues were observed occasionally. (2) Compared with rats of group C, the epilepsy in rats of group B showed obvious longer latencies and remarkably lower convulsion scores. No rat was dead in group B. While in group C 3 of 6 rats died from seizures. Latencies of seizures showed a tendency to become short. There was no obvious change of convulsion scores in group C. (3) The c-fos expression in group B significantly decreased at the same region of brain tissues when compared with that in group C ( P <0.01). Conclusion IVIG might play a role in the inhibition of convulsions and the reduction of the c-fos expressions.
出处
《中华儿科杂志》
CSCD
北大核心
2000年第6期358-360,共3页
Chinese Journal of Pediatrics
