摘要
目的观察镁锌合金(Mg-Zn)共同培养对成骨细胞MC3T3-E1的促增殖作用及相关的作用机制。方法实验分为对照组、Mg-Zn组、聚L-丙交酯(PLLA)组。采用细胞毒实验(MTT)法检测细胞增殖活性,酶联免疫分析(ELISA)法检测整合素β2的表达变化,免疫印迹法(Western blotting)检测细胞BMP-2和p-Smad1蛋白的表达变化。结果培养到第2天,3组间细胞增殖活性差异无统计学意义(P>0.05)。第4、6、8、10天时,Mg-Zn组MC3T3-E1细胞增殖显著高于对照组和PLLA组,差异有统计学意义(P<0.05);对照组与PLLA组细胞增殖活性比较,差异无统计学意义(P>0.05)。3组MC3T3-E1细胞整合素β2的表达在第2、4、6天逐渐升高,第8天又逐渐减低,差异有统计学意义(P<0.05),在同一时间点Mg-Zn组整合素β2的表达显著高于对照组和PLLA组,差异有统计学意义(P<0.01),对照组和PLLA组之间整合素β2的表达差异无统计学意义(P>0.05)。结论 Mg-Zn共培养可以促进成骨细胞的增殖,上调整合素β2的表达和激活BMP/Smad信号通路能是其主要的作用机制。
Objective To observe the effect of magnesium zinc（Mg-Zn） alloy on the proliferation of MC3T3-E1 osteoblasts,and to study its action mechanisms.Methods The treatment was divided into three group：control group,Mg-Zn alloy group,PLLA group.The activity of cells proliferation was measured by MTT analysis.The protein level of integrin β1 was determined by ELISA assay.Western blot was used to detect the protein expression of BMP-2 and p-Smad1,respectively.Results There were no significant difference among 3 groups in the second day（P0.05）;in the fourth,sixth,eighth and tenth day,the proliferation of MC3T3-E1 cells in Mg-Zn alloy group was significantly higher than that in control group and PLLA group（P0.05）;there were no statistics significance of the proliferation in control group and PLLA group（P0.05）;the expression of cell integrin β2 in 3 groups increased gradually in the second,fourth and sixth day and decreased gradually in the eighth day and showed statistic significance（P0.05）;at the same point of time,the expression of cell integrin β2 of Mg-Zn alloy were significantly higher than the that in control group and PLLA group（P0.01）;there were no statistic significance in the difference of the expression of cell integrin β2 between control group and PLLA group（P0.05）.Conclusion Mg-Zn alloy significantly promoted the proliferation of osteoblasts.The upregulation of the expression of integrin β2 and in stimulation of BMP/Smad pathway are the main action mechanisms.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第35期3732-3733,3736,共3页
Chongqing medicine
基金
国家自然科学基金资助项目(81000791)
