摘要
目的 :探讨在原发性食管鳞癌组织中染色体 9pter～ p2 1区域内的杂合性缺失及其意义。方法 :采用微卫星序列 PCR银染方法 ,检测 9pter～ p2 1区域内 3个位点的 DNA微卫星多态标记 ,并对 43例散发性原发食管鳞癌标本及其癌旁正常组织进行杂合性缺失分析。结果 :9pter～ p2 1区域内 3个 DNA微卫星多态标记的杂合性缺失率均大于 5 0 % ,有显著意义。结论 :在染色体 9pter～ p2
Objective:This present study was to determine the loss of heterozygosity(LOH) and its significance in 9pter^p21 of primary esophageal squamous cell carcinoma(ESCC).Methods:We have analyzed LOH in 9pter^p21 area in 43 sporadic primary ESCC paired samples of fresh frozen tumor and normal tissue. PCR silver stain method was used to examine the LOH of microsatellite polymorphic sequences at loci D9S324(9p23),D9S304(9p21.1),and D9S319(9p21.1).Results:The incidence of LOH in ESCC was 40 out of 62 informative cases. Moreover,LOH was detected at loci D9S324(77%),D9S304(63%),and D9S319(59%).Conclusion:The focuses for deletions in 9pter^p21 may exist both in 9p23 and in 9p21.1,which suggest that there are tumor suppressor genes(TSG) specific to esophageal squamous cell carcinoma in 9pter^p21.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2000年第1期27-28,共2页
Journal of China Medical University
关键词
杂合性缺失
食管鳞癌
9号染色体
食管癌
loss of heterozygosity
esophageal squamous cell carcinomas
chromosome 9
tumor suppressing gene
