摘要
目的探讨脆性组氨酸三联体基因(Fragile histidine triad,FHIT)杂合性丢失(Loss of heterozygosity,LOH)及人乳头瘤病毒(Human Papillomavirus,HPV)感染与宫颈癌的关系。方法选取FHIT基因的2个微卫星多态标记,采用聚合酶链反应(Polymerase chain reaction,PCR)法对50例宫颈癌及40例宫颈上皮内瘤样病变(Cervical in-traepithelial neoplasia,CIN)组织进行杂合性丢失及HPV16感染的检测。结果在D3S1234和D3S4103位点上宫颈癌的杂合性丢失发生率分别为46%(23/50)、40%(20/50);宫颈上皮内瘤样病变的杂合性丢失发生率分别为40%(16/40),37.5%(15/40)。宫颈癌与CIN在D3S1234位点上的LOH差异无统计学意义(P>0.05);在D3S4103位点上也无统计学意义(P>0.05)。但宫颈癌在2位点上LOH发生频率均高于CIN,且高级别CIN发生率高于低级别CIN。HPV16感染的阳性组中LOH发生率为79.5%(35/44);阴性组LOH发生率为33.3%(2/6),组间LOH的差异有统计学意义(P<0.05)。结论FHIT基因的变异可能发生在宫颈癌变的晚期;FHIT基因的LOH对于宫颈癌的筛查、早期诊断及判断预后可能具有临床实用价值。
Objective To explore file relationship of loss of heterozygosity of FHIT gene to the development of cervical carcinoma, and its relationship with HPV16 infection. Methods Two sites of microsatellite polyrnorphism of FHIT gene were selected, polymerase chain reaction（PCR） technique was used to detect LOH in 50 cases of cervical carcinoma and 40 cases of cervical intraepithelial neoplasia（CIN）, and to detect the infection state of HPV16. Results At D3S1234 and D3S4103, the LOH rates of cervical carcinomas were 46 % （23/50） and 40 % （20/50）, respectively. The LOH rates of CINs were 40 % （ 16/40 ） and 37.5 % （15/40）, respectively. There were no significant differences between cervical carcinomas and CINs in respect to their positive rates of LOH at D3S1234 and D3S4103 （P 〉 0.05）. The rates of LOH for cervical carcinomas were higher than those for CIN. The positive rates of LOH for HPV infenction were 79.5 % （35/44）;the negative rates of LOH were 33.3 % （2/6）, the incidence of LOH was higher in HPV （ ＋ ） than that in HPV （ - ）, P 〈 0.05. Conclusion The FHIT gene change is a. relatively late event in cervical carcinomas. The detection of the LOH of FHITgene might be helpful to the early diagnosis and the screening of cervical carcinoma. It might also be useful for predicting the prognosis of cervical carcinoma.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第12期1490-1491,共2页
Chinese Journal of Public Health
