摘要
目的:研究固相微萃取膜萃取条件对氯氮平提取效果的影响,建立固相微萃取膜技术提取氯氮平方法。方法:通过改变固相微萃取膜的膜浸泡吸附时间,酸性条件,超声时间,样品种类和浓度等,比较样品中氯氮平提取效果,选择最佳萃取条件,气相色谱法分析水样和尿样中氯氮平含量。结果:通过适当加大样品浓度、延长吸附时间,调节样品pH值为7,将超声技术引用到水样和尿样中膜解析过程,可有效提高固相微萃取膜对样品中氯氮平的提取率。结论:建立的水样和尿样中氯氮平的固相微萃取膜萃取方法,过程简单、检材及耗材用量少、提取率较高,杂质干扰少,可用于液体样品中氯氮平的提取。
Objective：To study the extraction efficiency of clozapine in water and urine by changing the extraction condition and establish a method of using solid-phase micro extraction membrane technology to extract clozapine.Methods： The extraction efficiency of clozapine in water and urine was analyzed by changing the extraction condition of solid-phase micro extraction membrane,such as the membrane adsorption time,acidity,supersonic time,sample type and concentration.Clozapine in water and urine were analyzed using GC.Results： The extraction efficiency of solid-phase micro extraction membrane technology for clozapine in water and urine were higher when sample solution pH was seven and clozapine concentration and immersion time of membrane increased,and the supersonic technology was used to resolve clozapine from membrane.Conclusion： The solid-phase micro extraction membrane method for clozapine in water and urine is simple with less sampling volume and impurity interference and higher extraction efficiency.It can be used for the determination of clozapine in liquid samples.
出处
《中国卫生检验杂志》
北大核心
2012年第11期2667-2669,共3页
Chinese Journal of Health Laboratory Technology
基金
山西省科技攻关项目(20110321082)
"十一五"国家科技支撑计划项目(2007BAK26B05)
关键词
氯氮平
固相微萃取膜
气相色谱法
Clozapine
Solid-phase micro extraction membrane
GC
