摘要
建立了基于微波辅助-顶空液相微萃取在线联用、高效液相色谱法测定水样中邻硝基苯酚的分析方法。采用L16(45)正交实验设计对影响萃取的各种因素,如萃取有机溶剂、微波辐射功率、萃取时间、离子强度、样品液体积,进行了优化。优化后萃取条件为,以乙酸丁酯作为萃取溶剂,功率和时间分别为100W和12min条件下,离子强度为0的样品溶液体积为20mL。在优化萃取条件下,邻硝基苯酚的检出限LOD(S/N=3)为0.94μg/L,萃取富集倍数为30,实际水样的加标回收率为85.2%。理论分析和实验结果表明,微波辅助-顶空液相微萃取在线联用方法具有简便、快速、高效、节省溶剂、选择性好、应用范围广的特点。
The method for the determination of 2-Nitrophenol in environmental water samples had been developed using microwave-assisted-headspace-liquid-phase microextraction（MA-HS-LPME） coupled with high performance liquid chromatography（HPLC）.The variable experimental conditions for the MA-HS-LPME extraction process,including extraction solvent,microwave irradiation power,extraction time,contents of inorganic salt and sample volume,were optimized by L16（45） orthogonal design.The optimized method was as following： butyl acetate as organic drop,20 mL aqueous sample without NaCl addition extracting for 12 min under microwave irradiation power 100 W.Under the optimized conditions,low detection limit（S/N=3） and the enrichment factor of the method for the target compound were 0.94μg/L and 30,respectively.The recoveries of target analyte spiked real water samples were 85.2%.The results indicated that the developed method is simple,rapid,efficient,solvent-saving,highly selective and widely applicable.
出处
《化学通报》
CAS
CSCD
北大核心
2011年第12期1140-1144,共5页
Chemistry
基金
重庆市教育委员会科学技术研究项目(KJ100102)
中央高校基本科研业务费项目(CDJXS10220004)资助
关键词
微波辅助萃取
顶空液相微萃取
高效液相色谱
邻硝基苯酚
Microwave-assisted extraction（MAE）
Headspace-liquid-phase microextraction（HS-LPME）
High performance liquid chromatography（HPLC）
2-Nitrophenol
