摘要
目的:建立GC-MS法测定动物性食品中15种兴奋剂残留量的新方法。方法:样品粉碎后经β-葡萄糖醛苷酶/芳基硫酸酯酶水解用乙酸钠缓冲溶液提取,SLW固相萃取柱净化,N,O双(三甲基硅基)三氟乙酰胺+三甲基氯硅烷(BSTFA+TMCS,99+1)衍生化。采用选择离子监控模式(SIM)检测,GC/MS测定测定其含量。结果:15种β-兴奋剂不同水平的加标回收率范围为76.2%～101.4%,相对标准偏差为2.65%～9.53%。结论:本方法选择性和灵敏度高,实用性强。
Objective：Developing a method to determine 15 β-agonists in animal food by GC-MS（gas chromatograph mass spectrometer）.Methods： The homogenized samples were digested byβ-glucuronidase /aryl sulfatase,and extracted with sodium acetate.Further cleanup was performed on a SLW cartridge and the purified drugs were derivatized with [N,O-bis（trimethylsilyl） trifluoroacetamide（BSTFA）-trimethylchlorosilane（TMCS）,99＋1],（SIM） method was applied for detection.Results： The average recovery were 76.2%~101.4%.The relative standard deviations were 2.65%~9.53%.Conclusion： The selectivity and the sensitivity of the method is high,the method is practical.
出处
《中国卫生检验杂志》
北大核心
2012年第11期2544-2545,2549,共3页
Chinese Journal of Health Laboratory Technology
基金
广州市医药卫生科技项目(201102A213057)
