摘要
目的 评价单唾液酸西己糖神经糖甙脂钠盐 (Monosialotetrahexosylganglioside ,GM1)在流体冲击致大鼠脑损伤 (Fluid percussionbraininjury ,FPBI)后急性期对大脑的保护作用 ,以期为脑损伤的临床治疗提供实验依据。方法 流体冲击致大鼠闭合型脑损伤后 ,用干湿法测定脑组织水含量、氢清除法观测局部脑血流量 (rCBF)的改变 ,以及病理改变。结果 大鼠脑损伤后GM1能显著降低伤后水含量的升高 ;伤后rCBF未见明显降低 ,同时病理学检查GM1明显减轻病理学改变。结论 早期应用GM1能有效地阻止rCBF的缺血 /再灌流变化 ,改善脑组织血供从而有效阻止脑水肿、神经元变性。
Objective To evaluate the protective effect of monosialotetrahexosylganglioside (GM1) on the acute stage of fluid percussion brain injury (FPBI) in rats to provide experimental basis for the clinical treatment. Methods After the establishment of rat model of FPBI, the water content of brain tissues was detected with dry wet method and regional cerebral blood flow (rCBF) was measured with H 2 elimination method. Pathological changes of the brain were observed with light microscope. Results GM1 significantly inhibited the elevation of the water content in the brain and showed no effect on the rCBF after FPBI. Meanwhile, GM1 significantly ameliorated the pathological manifestation of the brains. Conclusion Early administration of GM1 can prevent ischemia/reperfusion damages of rCBF, improve cerebral blood supply and reduce cerebral edema, denaturation and necrosis of the neurons after fluid percussion brain injury in rats.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第6期585-587,共3页
Journal of Third Military Medical University
关键词
流体冲击
大鼠
颅脑损伤
脑保护
GM1
monosialotetrahexosylganglioside
fluid percussion
brain injury
rat
