摘要
ADP核糖基化因子(Arf)GTP酶激活蛋白(GAPs)能够促使束缚于Arfs的GTP水解为GDP,通过转化具有活性的GTP束缚型为非活性的GDP束缚型从而达到调节Arfs的作用。本研究从辣椒幼苗叶片cDNA文库中筛选获得1个阳性克隆。核苷酸序列分析表明,该基因编码含有1个408个氨基酸残基,分子量约为43.98kD,含有1个保守的Arf GAP结构域,与葡萄、大豆中的AGD8-Like基因的同源性分别为73%和71%,与拟南芥AtAGD8同源性为65%,命名为CaAGD8。实时荧光定量PCR检测表明,与对照相比,CaAGD8基因的相对表达量在辣椒茎、叶、花及果实中表达量升高。
ADP ribosylation factor（Arf） GTPase-activating proteins（GAPs） promote the hydrolysis of GTP bound to Arfs to GDP,which plays a pivotal role in regulating Arfs by converting the active GTP-bound forms of these proteins into their inactive GDP-bound forms.The positive clone was screened from was screening and isolated from cDNA library of seedling leaves of pepper and was designated as CaAGD8.Sequence analysis showed that the 43.98kD proteins encoded a polypeptide of 408 amino acid residues and contain an Arf GAP domain.The CaAGD8 with 73% and 71% similarity respectively to the AGD8-Like of grape and soybean and with 65% similarity to the AtAGD8.The real time PCR results showed that compared with control CaAGD8 relative expression was higher in stems,leaves,flowers and fruits of pepper.
出处
《中国农学通报》
CSCD
2012年第34期184-189,共6页
Chinese Agricultural Science Bulletin
基金
高校博士点基金"辣椒NAC家族成员的分离及其功能研究"(20093515110004)
福建省自然基金重点项目"CaWRKY5激活植物低温抗性的结构基础和分子机制研究"(2008J0003)
关键词
辣椒
ARF
GAP
表达
Capsicum annuum L.
Arf GAP
expression
