摘要
从麻疯树cDNA文库中筛选到包含完整编码序列的ADP核糖基化因子的cDNA序列,命名为Jc-arf。其长度为887bp,开放阅读框546bp,推测的编码蛋白含181个氨基酸残基,具有典型的GTP结合蛋白家族的特点:P框(GLDAAGKT)、G’框(DVGGQ)和G框(NKQDL)。序列分析显示,Jc-arf接近于人类ClassI型arf基因,其蛋白序列与多种植物ARF有很高的同源性。半定量RT-PCR结果显示,Jc-arf的表达具有一定的组织特异性,在花中最丰富。PEG、低温、NaCl胁迫下,Jc-arf的表达受PEG和低温的诱导,而对NaCl胁迫不敏感。
An ADP-ribosylation factor gene, named Jc-arf, was cloned from Jatropha curcas. The cloned eDNA included a 546 bp complete open reading frame, which encoded a 181 amino acids peptid. This putative peptid contained three motifs： GLDAAGKT, DVGGQ and NKQDL, which involved in the feature of GTP-binding proteins. The alignment results showed Jc-ARF was high identity with many ARFs in other plants, and it was similar to ClassI of human ARFs. The semi-quantitative PCR results showed that Jc-arfexpression was the highest in flowers. Further, the expression of Jc-arf could be induced in roots, stems and leaves by PEG, in roots under cool stress. However, it seemed insensitive to the NaCl stress.
出处
《植物生理学通讯》
CAS
CSCD
北大核心
2009年第6期549-554,共6页
Plant Physiology Communications
基金
国家自然科学基金(30670204)
科技部国际合作项目(2006DFB63400)
"十一五"科技支撑项目(2006BAD07A04)
关键词
麻疯树
ADP核糖基化因子
表达
胁迫
Jatropha curcas
ADP-ribosylation factor
expression
stress
