摘要
作者建立了大鼠气管上皮(rat tracheal epithelial,RTE)细胞的单层原代培养和RTE细胞体外转化实验,比较有滋养层和无滋养层RTE细胞转化系统的实验结果。首次证明烟草中的特殊亚硝胺——甲基亚硝基吡啶基丁酮(NNK)对RTE细胞有体外转化作用。在甲基硝基亚硝基胍(MNNG)和NNK诱导转化实验中,有滋养层和无滋养层体外转化系统得到的结果一致,而后者在RTE细胞体外转化应用中更为敏感而方便。
An in vitro transformation system was established using primary rat tracheal epithelial (RTE)cells cultured in medium with or without feeder layer cells. About 24～48 hours after plating, RTE cells were exposed to N-methyl-N '-nitro-N-nitrosoguanidine (MNNG, 0～0.6μg/ml), benzo (a) pyrene (BaP, 0～20μg/ml), or 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK, 0～2.0mg/ml). One or two weeks after exposure, enhanced growth variants (EGV)among the RTE cells were selected in serumcontaining medium by removing either the feeder layer or bovine pitutary extract (BPE) and epidermal growth factor (EGF). The results showed that EGV transformation was induced by MNNG, BaP and NNK, and a dose dependent EGV transformation was observed in the MNNG and NNK experiments.Our experimental results suggest that for the selection of EGV's from among RTE cellsexposed to chemical carcinogens, the use of BP E-and EGF-free medium is more sensitive and convenient than is removing the feeder layers.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1990年第5期325-329,T011,T012,共7页
Acta Academiae Medicinae Sinicae
关键词
气管上皮细胞
体外转化
化学致癌物
tracheal epithelial cells in vitro transformation chemical carcinogens
