摘要
目的 探讨更生霉素对氩绿激光诱导的SD大鼠视网膜组织损伤的抑制作用。方法大鼠双眼激光照射后即刻、8、2 0h分别于玻璃体腔内注射不同浓度的更生霉素 (10g/L、5g/L、2 5g/L) ,对照眼注射生理盐水 ,激光后 2 4h取双眼眼球 ,视网膜组织切片行甲基绿 哌洛宁染色 ,计数每个激光斑皱缩细胞、正常细胞、总细胞数。结果 更生霉素可明显降低激光斑处的皱缩细胞及累及的总细胞数 ,实验组与对照组有显著差别 ,以用药后即刻及 8h作用最明显 ,随给药时间的延后 ,药物浓度的增加并不能增强抗损伤作用。结论 更生霉素有抗激光损伤的作用 。
Objective To determine that the administration of RNA synthesis inhibitor (dactinomycin) prevents retinal photoreceptor cell injury induced by green wavelength of argon laser in SD mice Methods At various time (0, 8, 20 h) after the exposure to laser, eyes of the mice were injected intraocularly with dactinomycin in experimental eyes, and saline in control ones, and the concentration of the drug were 10 g/L, 5 g/L, 2 5 g/L The eyes were taken at 24 h after the exposure, the slices of the retina were stained with methyl green pyronin (MG P), and pyknotic (violet) as well as surviving cells (green) were counted in the laser burns Results Intraocular administration of dactinomycin diminished the degeneration induced by argon laser, there were marked differences between the experimental group and the control group, dactinomycin showed to be more effective if administered immediately after exposure and 8 h later, after this time the higher concentration of the drug could not result in higher effect Conclusion Dactinomycin can inhibit the occurrence of the laser injury, but the time selected for the administration is critical for its efficacy
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2000年第4期299-301,共3页
Chinese Journal of Ophthalmology
基金
军队‘九五’医药卫生科研基金!( 96M14 3 )
