摘要
目的 探讨一氧化碳 (CO)在急性呼吸窘迫综合征 (ARDS)发病中的变化及其意义。方法 通过尾静脉注射油酸 (OA)制备大鼠ARDS模型 ,测其不同病程时肺动脉、颈动脉、颈静脉血和支气管肺泡灌洗液 (BALF)中CO含量变化 ,同时测定一氧化氮 (NO)水平。结果 给大鼠静脉注射OA0 5h ,其肺动脉血CO水平即见增加 ,2h显著高于对照组 ,也高于同一时间点颈动脉血和静脉血 ,此后一直维持于较高水平 ;0 5～ 2 4hBALF中可检出CO。注射OA后 0 5、2hARDS大鼠肺动脉血、颈动脉血和颈静脉血浆中NO均明显升高 ,6h开始下降 ,12h后接近正常水平 ;BALF中NO显著高于对照组。结论 给大鼠静脉注射OA可引起肺循环血中CO水平增加 。
Objective To explore the possible involvement of carbon monoxide (CO) in the development of oleic acid (OA) induced ARDS. Methods We determined the blood concentration of CO in pulmonary artery, carotid artery and jugular vein, as well as the concentration of CO in bronchoalvcolar lavage fluid (BALF)from male SD rats 0.5, 2, 6, 12, 24, 72 h after injection with OA via caudal vein. The plasma concentration of nitric oxide (NO) in the same samples as above was detected at the sametime. Results There was a tendency of increase of pulmonary artery CO in rats 0.5 h after OA injection.The pulmonary artery CO was significantly higher in the rats at 2 h after OA injection than in control rats (0.135±0.010) g/L versus (0.116±0.005) g/L, P <0.01. It was also higher than that in carotid artery (0.117±0.013) g/L and jugular vein (0.107±0.018) g/L in the same group of rats and maintained a relatively high level thereafter. CO was detected in BALF of all the rats 0.5～24 h after OA injection, but in none of the controls and the 72 h group. The plasma levels of NO in pulmonary and systemic circulation were all markedly elevated in rats 0.5, 2 h after OA injection, and then sharply declined. The BALF NO in all the OA injection rats was significantly higher than that in the control rats. Conclusion It is suggested that increased concentration of CO in pulmonary artery might play an important role that differs from NO in the pathogenesis of ARDS.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2000年第6期388-391,共4页
Chinese Journal of Internal Medicine
基金
国家自然科学基金! ( 3 9770 63 8)
国家教育部博士点基金! ( 982 2 )
