摘要
目的:观察鬼针草总黄酮对大鼠内毒素性急性肺损伤(ALI)的抗炎和抗氧化作用,探讨其对ALI保护作用的机制。方法:120只大鼠随机分为正常对照组(生理盐水),模型组,地塞米松组(5 mg.kg-1,ip),鬼针草总黄酮低、中、高剂量组(100,150,200 mg.kg-1,ip),每组20只。给药3 d,末次ip给药1 h后,ip内毒素诱导ALI模型,6 h后处死,观察每组肺泡灌洗液白细胞和蛋白含量、肺组织核转录因子(NF-κB)p65表达以及肿瘤坏死因子(TNF-α)、白细胞介素(IL-6)、白细胞介素(IL-10)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量以及中性粒细胞髓过氧化物酶(MPO)活性。结果:与对照组比较,模型组的肺泡灌洗液白细胞、蛋白含量显著增加(P<0.05～P<0.01);鬼针草总黄酮中、高剂量组肺组织NF-κB p65表达为(38.46±4.56)%,(31.39±4.18)%,MDA含量为(1.73±0.19),(1.46±0.15)nmol.mg-1,TNF-α含量为(259.33±37.48),(211.46±32.69)μg.L-1,IL-6含量为(287.46±100.18),(223.55±93.49)ng.L-1,MPO活性为(2.69±0.57),(2.43±0.38)U.g-1,与模型组比较均明显降低(P<0.05～P<0.01);IL-10含量为(16.73±3.87),(17.28±3.58)μg.L-1,SOD为(69.46±9.84,73.24±8.39)U.mg-1,与模型组比较均明显升高(P<0.05～P<0.01)。结论:鬼针草总黄酮能减轻LPS诱发急性肺损伤所致肺血管通透性,减少炎症渗出,降低氧化应激损伤程度。
Objective： To investigate anti-inflammatory and antioxidant function of total flavones of Bidens bipinnata L （TFB） on lipoplysaccharide （LPS） -induced acute lung injury （ALI） and the mechanism. Method： One hundred and twenty SD rats were randomly divided into six groups： control group （physiological saline, ip）, model group, dexamethasone group （5 mg ·kg^-1, ip） , TFB treatment group （100, 150, 200 mg ·kg^-1, ip） , twenty rats in each group. The rat ALI model was induced by LPS, 5 mg·kg^-1 , ip. Six hours after modeling, all rats were sacrificed, protein content, white blood cell in bronehoalveolar lavage fluid （BALF） , expression of NF-κB. p65, and level of tumor necrosis factor （TNF-α）, interleukin-6 （ IL-6）, interleukin-lO （IL-10） superoxide dismutase （SOD）, malondialdehyde （MDA） and the activity of myeloperoxidase （MPO） in lung were measured. Result： Compared with control group, content of WBC and the protein in BALF was higher （P〈 0.05-P 〈 0.01）, compared with model group, NF-KB p65 expression level was （38.46 ± 4.56）%, （31.39 ±4. 18）% （in TFB high and medium dose group, respectively）, the content of MDA was （1.73 0.19）, （1.46±0.15） nmol·mg^-1, and the content of TNF-α was （259.33±37.48）, （211.46±32.69） μg ·L^-1, and the content of IL-6 was （287.46 ±100. 18）, （223.55± 93.49） ng ·L^-1, and the activitiy of MPO was （2.69 ±0.57） , （2.43 ±0.38） U ·g^-1 respectevly. NF-κB p65 expression level and the content of MDA TNF-α, IL-6 and the activities of MPO in lung were significantly lower （P 〈 0.05-P 〈 0.01 ）. On the other hand, the level of IL-10 was （16.73 ±3.87）, （17.28 ±3.58） μg·L^-1 the content of SOD was （69.46 ±9.84） （73.24±8.39） U·mg^-1, the content of IL-10 and SOD was increased significantly （P 〈0. 05-P 〈0.01）. Conclusion： TFB can reduce pulmonary vascular permeability of LPS induced acute lung injury, reduce the inflammatory exudation and oxidative stress damage.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第24期265-268,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
鬼针草总黄酮
急性肺损伤
脂多糖
炎性因子
氧化应激
total flavones of Bidens bipinnata （TFB）
acute lung injury
lipopolysaecharide
inflammatory furor
oxidative stress
