摘要
采用食下毒叶法、药膜法和点滴法测定丁烯氟虫腈对家蚕的急性毒性,并进行了急性风险评价。食下毒叶法结果表明丁烯氟虫腈对蚁蚕、2龄、3龄、4龄和5龄起蚕的摄入LC50值(96h,下同)分别为280(227～343)、578(507～652)、3612(3178～4139)、6790(6045～7875)、7151(5932～8705)mg·L-1,均大于200mg·L-1,属于低毒级;桑叶浸药时间为1s、10s、1min、10min和60min时,丁烯氟虫腈对2龄起蚕的LC50值分别为789(690～913)、578(507～652)、443(382～499)、396(349～447)、254(222～285)mg·L-1;家蚕在丁烯氟虫腈药膜上爬行10、30和60min后,对2龄起蚕的接触LC50值分别为101(86.8～131)、66.7(61.7～74.1)、60.9(51.0～83.4)μg·cm-2,对3龄起蚕的点滴接触LD50值为76.2(70.2～84.9)μg·larva-1。急性风险评价结果表明,丁烯氟虫腈对蚁蚕具有中等风险性,对其他龄期家蚕为低风险。因此,丁烯氟虫腈对家蚕的急性毒性和急性风险均较低。
Acute toxicities of butene-fipronil to silkworm （Bombyx mori）in different situations were evaluated by the methods of feeding toxic mulberry leaves, methods of topical application on the pronotum and contacting with toxic membrane, respectively. And the risk assessment for butene-fipronil was also made. For the first, second, third, fourth and fLfth-instar larvae, the 96 h-LCs0 values of butene-fipronil to silkworm were 280 （227-343）, 578 （507-652）, 3612 （3178-4139）, 6790 （6045-7875）, 7151 （5932-8705） mg·L -1, respectively, which were higher than 200 mg·L -1, belonging to low-level toxicity. When immersion time of mulberry leaves in butenefipronil solution was 1 s, 10 s, 1 min, 10 min and 60 rain, the 96 h-LC50 values to the second - instar larvae were 789 （690-913）, 578 （507-652）, 443 （382-499）, 396 （349-447）, 254 （222-285） mg·L -1, respectively. After 10, 30 and 60 rain contact of silkworm with filter paper treated by butene-fipronil solution, the 96 h-LC50 values to the seeond-instar larvae were 101 （86.8-131）, 66.7 （61.7-74.1）, 60.9 （51.0-83.4）μg. cm 2, respectively. The 96 h-LDs0 value by topical application on the pronotum to the third-instar larvae was 762 （70.2-84.9） μg·larva -1. The acute risk assessment results showed that the acute risk of butene-fipronil to the first-instar larvae was intermediate and those of butene-fipronil to other instar larvae were low. It is indicated that the acute toxicity and risk of butene-fipronil to silkworm are both low.
出处
《生态毒理学报》
CAS
CSCD
北大核心
2012年第6期639-645,共7页
Asian Journal of Ecotoxicology
基金
科技部863项目(2011AA100806)
科技部国际合作项目(S2010GR0905)
浙江省农业科学院院选项目(2010R28Y01E01)
关键词
丁烯氟虫腈
家蚕
急性毒性
急性风险评价
butene-fipronil
Bombyx mori
acute toxicity
acute risk assessment
